{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T07:08:28Z","timestamp":1763017708564,"version":"3.41.2"},"reference-count":57,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,2,9]],"date-time":"2017-02-09T00:00:00Z","timestamp":1486598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Dairy Research Institute, USA"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Whey protein isolate (WPI) contains vitamin B<jats:sub>12<\/jats:sub> and folate. However, the efficacy of WPI as a bioavailable source of these vitamins in the elderly with low vitamin B<jats:sub>12<\/jats:sub> was not previously tested. We investigated the effects of WPI supplementation on vitamin B<jats:sub>12<\/jats:sub> and folate status in blood and measured changes in homocysteine (HCY), methylmalonic acid (MMA), and genome integrity biomarkers in elderly individuals with low vitamin B<jats:sub>12<\/jats:sub> status. The effect of WPI was compared to soy protein isolate (SPI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>In this randomized controlled cross\u2010over intervention trial, 56 subclinically vitamin B<jats:sub>12<\/jats:sub>\u2010deficient participants received 50 g WPI or 50 g SPI as a control for 8 wk followed by 16\u2010wk washout phase and then cross\u2010over to alternative supplement for next 8 wk. Consumption of WPI resulted in significant increase in serum active B<jats:sub>12<\/jats:sub> (<jats:italic>p<\/jats:italic> &lt; 0.0001) and serum folate (<jats:italic>p<\/jats:italic> = 0.0094). MMA, HCY, and nucleoplasmic bridges increased significantly after SPI intake but not after WPI (<jats:italic>p<\/jats:italic> = 0.052; <jats:italic>p<\/jats:italic> = 0.028; <jats:italic>p<\/jats:italic> = 0.0009, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Results indicate that WPI consumption improves active B<jats:sub>12<\/jats:sub> and folate status. Unlike SPI, WPI consumption may prevent increase in MMA, HCY, and genome instability in older Australians with low vitamin B<jats:sub>12<\/jats:sub> status.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201600915","type":"journal-article","created":{"date-parts":[[2016,12,17]],"date-time":"2016-12-17T14:38:14Z","timestamp":1481985494000},"source":"Crossref","is-referenced-by-count":16,"title":["Whey protein isolate improves vitamin B<sub>12<\/sub> and folate status in elderly Australians with subclinical deficiency of vitamin B<sub>12<\/sub>"],"prefix":"10.1002","volume":"61","author":[{"given":"Varinderpal S.","family":"Dhillon","sequence":"first","affiliation":[{"name":"Genome Health Commonwealth Scientific and Industrial Research Organisation Health and Biosecurity  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrios","family":"Zabaras","sequence":"additional","affiliation":[{"name":"Genome Health Commonwealth Scientific and Industrial Research Organisation Health and Biosecurity  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodora","family":"Almond","sequence":"additional","affiliation":[{"name":"Genome Health Commonwealth Scientific and Industrial Research Organisation Health and Biosecurity  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Cavuoto","sequence":"additional","affiliation":[{"name":"Genome Health Commonwealth Scientific and Industrial Research Organisation Health and Biosecurity  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Genevieve","family":"James\u2010Martin","sequence":"additional","affiliation":[{"name":"Genome Health Commonwealth Scientific and Industrial Research Organisation Health and Biosecurity  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Fenech","sequence":"additional","affiliation":[{"name":"Genome Health Commonwealth Scientific and Industrial Research Organisation Health and Biosecurity  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000337775"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2011.11.003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1041610211002511"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2005.07.018"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1586\/ern.09.75"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.7.14.7901104"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/84.6.1406"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12603-010-0013-1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3174\/ajnr.A3785"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.115.116970"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2015.10.003"},{"volume-title":"Adams and Victor's Principles of Neurology","year":"2001","author":"Victor M.","key":"e_1_2_8_13_1"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2014.09.011"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-2199-9_17"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.7.3290"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2010.02.004"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(97)76230-1"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000325581.26991.f2"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2008.26598"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/80.6.1500"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(95)00173-5"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511002364"},{"key":"e_1_2_8_24_1","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1128\/am.14.3.353-355.1966","article-title":"Use of cheese whey for vitamin B12 production. 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