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Egg white ovotransferrin\u2010derived tripeptides, IRW (Ile\u2010Arg\u2010Trp), IQW (Ile\u2010Gln\u2010Trp), or LKP (Leu\u2010Lys\u2010Pro) are previously identified as the inhibitors of angiotensin\u2010converting enzyme (ACE), a key enzyme in the RAS. This study aims at determining whether these peptides are effective in improving insulin resistance, and their mechanisms of action, in a rat derived skeletal muscle cell line (L6 cells).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Insulin resistance is induced by treating L6 cells with 1 \u03bc<jats:sc>m<\/jats:sc>angiotensin II (Ang II) for 24 h. Effects of peptides on glucose uptake are determined using glucose uptake assay, glucose transporter 4 (GLUT4) translocation by immunofluorescence, reactive oxygen species (ROS) by dihydroethidium (DHE) staining, while insulin signaling pathway, Ang II receptor (AT1R or AT2R) levels, and NADPH oxidase activation are measured using Western Blot. Only IRW treatment significantly improves insulin resistance in L6 cells via stimulation of insulin signaling. IRW decreases Ang II\u2010stimulated AT1R expression, ROS formation, and NADPH oxidase activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Of three ACE inhibitory peptides studied, only IRW improves insulin resistance in L6 cells, at least partially via reduced AT1R expression and its anti\u2010oxidative activity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201700602","type":"journal-article","created":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T11:22:42Z","timestamp":1514287362000},"source":"Crossref","is-referenced-by-count":41,"title":["Egg White Ovotransferrin\u2010Derived ACE Inhibitory Peptide Ameliorates Angiotensin II\u2010Stimulated Insulin Resistance in Skeletal Muscle Cells"],"prefix":"10.1002","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8480-5169","authenticated-orcid":false,"given":"Myoungjin","family":"Son","sequence":"first","affiliation":[{"name":"Department of Agricultural Food &amp; Nutritional Science University of Alberta Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine B.","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Physiology University of Alberta Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Agricultural Food &amp; Nutritional Science University of Alberta Edmonton Alberta Canada"},{"name":"Cardiovascular Research Centre University of Alberta Edmonton Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,29]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"International Diabetes Federation.https:\/\/www.idf.org\/our-activities\/advocacy-awareness\/resources-and-tools\/60:idfconsensus-worldwide-definitionof-the-metabolic-syndrome.html. 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