{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T14:58:57Z","timestamp":1791644337018,"version":"4.3.4"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,25]],"date-time":"2018-01-25T00:00:00Z","timestamp":1516838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Sunstar Inc."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Hypertension is a risk factor for arteriosclerosis. In this study, we investigate the antihypertensive effect of protease\u2010digested rice bran in a spontaneously hypertension rat (SHR) model. We also purify a novel antihypertensive peptide from the digest.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Thermolysin\u2010digested rice bran (TRB) is administered to SHRs for 4 weeks, and systolic blood pressure (SBP) was measured weekly using the tail\u2010cuff method. TRB shows an antihypertensive effect in a dose\u2010dependent manner. TRB also reduces angiotensin I\u2010converting enzyme (ACE) activity in lung tissue and serum troponin I levels. TRB is fractionated by HPLC and ACE\u2010inhibitory activity in the HPLC fractions is measured. Peptides LRA and YY are identified from the two fractions with the strongest ACE\u2010inhibitory activity. Amino acid sequence of these peptides are found in a vicilin\u2010like seed storage protein, and identified in rice bran protein using the peptide mass fingerprint method. We confirm that LRA and YY are cleaved by thermolysin digestion of a model synthetic peptide. Orally administered LRA (0.25\u00a0mg\u00a0kg<jats:sup>\u22121<\/jats:sup>) or YY (0.5\u00a0mg\u00a0kg<jats:sup>\u22121<\/jats:sup>) lowers the SBP of SHRs at 4\u00a0h after administration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We identify a novel, orally active antihypertensive peptide, LRA from the digest of rice bran protein.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201700732","type":"journal-article","created":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T16:52:39Z","timestamp":1514393559000},"source":"Crossref","is-referenced-by-count":69,"title":["A Novel Antihypertensive Peptide Identified in Thermolysin\u2010Digested Rice Bran"],"prefix":"10.1002","volume":"62","author":[{"given":"Naohisa","family":"Shobako","sequence":"first","affiliation":[{"name":"Health Care R&amp;D Sunstar Group Takatsuki Osaka Japan"},{"name":"Division of Food Science and Biotechnology Graduate School of Agriculture Kyoto University Uji Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaro","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Health Care R&amp;D Sunstar Group Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Ishikado","sequence":"additional","affiliation":[{"name":"Health Care R&amp;D Sunstar Group Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayo","family":"Harada","sequence":"additional","affiliation":[{"name":"Health Care R&amp;D Sunstar Group Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etsuko","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Health Care R&amp;D Sunstar Group Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirohisa","family":"Suido","sequence":"additional","affiliation":[{"name":"Health Care R&amp;D Sunstar Group Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Kusakari","sequence":"additional","affiliation":[{"name":"Health Care R&amp;D Sunstar Group Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariko","family":"Maeda","sequence":"additional","affiliation":[{"name":"Health Care R&amp;D Sunstar Group Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Suwa","sequence":"additional","affiliation":[{"name":"Health Care R&amp;D Sunstar Group Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motonobu","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Health Care R&amp;D Sunstar Group Takatsuki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuhei","family":"Kanamoto","sequence":"additional","affiliation":[{"name":"Division of Food Science and Biotechnology Graduate School of Agriculture Kyoto University Uji Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kousaku","family":"Ohinata","sequence":"additional","affiliation":[{"name":"Division of Food Science and Biotechnology Graduate School of Agriculture Kyoto University Uji Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10717544.2016.1255999"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161207780363068"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.70.1118"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.56.1541"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf020482t"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.12540"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2015.12.033"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2014.09.107"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3136\/fstr.6.208"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2015.06.057"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11746-011-1941-6"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-008-0945-4"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2016.08.047"},{"key":"e_1_2_8_15_1","author":"Senaphan K.","year":"2016","journal-title":"Eur. 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