{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:45:51Z","timestamp":1790430351204,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T00:00:00Z","timestamp":1532476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>The secretion of gut hormones, such as cholecystokinin (CCK) is stimulated by fatty acids. Although a chain length\u2013dependent mechanism has been proposed, other structural relationships to releasing activity remain unclear. We aimed to elucidate specific structures in fatty acids that are responsible for their CCK\u2010releasing activity, and related sensing mechanisms in enteroendocrine cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>CCK secretory activities were examined in a murine CCK\u2010producing cell line STC\u20101 by exposing the cells to various modified fatty acids produced by gut lactic acid bacteria. The effects of fatty acids on gastric emptying rate as a CCK\u2010mediated function were examined using acetaminophen and phenol red methods in rats. Out of more than 30 octadecanoic\u2010derived fatty acids tested, 5 oxo\u2010fatty acids potently stimulated CCK secretion without cytotoxic effects in STC\u20101 cells. Three fatty acids had a distinct specific structure containing one double bond, whereas the other two had two double bonds, nearby an oxo residue. CCK secretion induced by representative fatty acids (10\u2010oxo\u2010<jats:italic>trans<\/jats:italic>\u201011\u201018:1 and 13\u2010oxo\u2010<jats:italic>cis<\/jats:italic>\u20109,<jats:italic>cis<\/jats:italic>\u201015\u201018:2) was attenuated by a fatty acid receptor G\u2010protein coupled receptor 40 antagonist. Oral administration of 13\u2010oxo\u2010<jats:italic>cis<\/jats:italic>\u20109,<jats:italic>cis<\/jats:italic>\u201015\u201018:2 lowered the gastric emptying rate in rats in a dose\u2010 and structure\u2010dependent manner.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results reveal a novel fatty acid\u2010sensing mechanism in enteroendocrine cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201800146","type":"journal-article","created":{"date-parts":[[2018,6,25]],"date-time":"2018-06-25T11:49:25Z","timestamp":1529927365000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Novel Mechanism of Fatty Acid Sensing in Enteroendocrine Cells: Specific Structures in Oxo\u2010Fatty Acids Produced by Gut Bacteria Are Responsible for CCK Secretion in STC\u20101 Cells via GPR40"],"prefix":"10.1002","volume":"62","author":[{"given":"Tohru","family":"Hira","sequence":"first","affiliation":[{"name":"Research Faculty of Agriculture Hokkaido University  Sapporo 060\u20108589 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shono","family":"Ogasawara","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture Hokkaido University  Sapporo 060\u20108589 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asuka","family":"Yahagi","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture Hokkaido University  Sapporo 060\u20108589 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minami","family":"Kamachi","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture Hokkaido University  Sapporo 060\u20108589 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Li","sequence":"additional","affiliation":[{"name":"School of Agriculture Hokkaido University  Sapporo 060\u20108589 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saki","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Fundamental Research Laboratory, Research and Development Division J\u2010Oil Mills, Inc.  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