{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:13:05Z","timestamp":1790709185591,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T00:00:00Z","timestamp":1537488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31502024"],"award-info":[{"award-number":["31502024"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007540","name":"Jiangsu Agricultural Science and Technology Innovation Fund","doi-asserted-by":"publisher","award":["CX[15]1066"],"award-info":[{"award-number":["CX[15]1066"]}],"id":[{"id":"10.13039\/100007540","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>The intestinal mucosal barrier, including the mucus layer, protects against invasion of enteropathogens, thereby inhibiting infection. In this study, the protective effect of <jats:italic>Lactobacillus<\/jats:italic> on the intestinal barrier against <jats:italic>Salmonella<\/jats:italic> infection is investigated. The underlying mechanism of its effect, specifically on the regulation of goblet cells through the Notch pathway, is also elucidated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Here, the protective effect of <jats:italic>Lactobacillus<\/jats:italic> on alleviating changes in the intestinal barrier caused by <jats:italic>Salmonella<\/jats:italic> infection is explored. It has been found that <jats:italic>Salmonella typhimurium<\/jats:italic> colonizes the colon and damages colonic mucosa. However, <jats:italic>Lactobacillus acidophilus<\/jats:italic> ATCC 4356 alleviates the colitis caused by <jats:italic>Salmonella<\/jats:italic> infection. Moreover, <jats:italic>S. typhimurium<\/jats:italic> infection causes colonic crypt hyperplasia with increased PCNA<jats:sup>+<\/jats:sup> cells, while <jats:italic>L. acidophilus<\/jats:italic> administration resolves these pathological changes. In addition, it has been further demonstrated that <jats:italic>Salmonella<\/jats:italic> results in severe colitis associated with goblet cells, and <jats:italic>Lactobacillus<\/jats:italic> improves colitis similarly associated with goblet cells. <jats:italic>Salmonella<\/jats:italic> infection induces goblet cell loss and reduces MUC2 expression by increasing Dll1, Dll4, and HES1 expression, while <jats:italic>L. acidophilus<\/jats:italic> reverses epithelial damage by balancing the Notch pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The study demonstrates that colitis improvement is controlled by <jats:italic>Lactobacillus<\/jats:italic> ATCC 4356 by regulation of the Notch pathway; this finding will be useful for prevention against animal <jats:italic>S. typhimurium<\/jats:italic> infection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201800552","type":"journal-article","created":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T08:56:34Z","timestamp":1536569794000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["<i>Lactobacillus acidophilus<\/i> Alleviated <i>Salmonella<\/i>\u2010Induced Goblet Cells Loss and Colitis by Notch Pathway"],"prefix":"10.1002","volume":"62","author":[{"given":"Haiqin","family":"Wu","sequence":"first","affiliation":[{"name":"MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary Medicine Nanjing Agricultural University  No. 1 Weigang Nanjing Jiangsu 210095 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Ye","sequence":"additional","affiliation":[{"name":"MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary Medicine Nanjing Agricultural University  No. 1 Weigang Nanjing Jiangsu 210095 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxi","family":"Lu","sequence":"additional","affiliation":[{"name":"MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary Medicine Nanjing Agricultural University  No. 1 Weigang Nanjing Jiangsu 210095 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Xie","sequence":"additional","affiliation":[{"name":"MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary Medicine Nanjing Agricultural University  No. 1 Weigang Nanjing Jiangsu 210095 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Yang","sequence":"additional","affiliation":[{"name":"MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary Medicine Nanjing Agricultural University  No. 1 Weigang Nanjing Jiangsu 210095 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1168-4132","authenticated-orcid":false,"given":"Qinghua","family":"Yu","sequence":"additional","affiliation":[{"name":"MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary Medicine Nanjing Agricultural University  No. 1 Weigang Nanjing Jiangsu 210095 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2653"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat0835"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2016.169"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2017.10.049"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.07.008"},{"key":"e_1_2_7_7_1","author":"Martens E. C.","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.58"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.88"},{"key":"e_1_2_7_10_1","first-page":"4","volume":"15","author":"Dickson I.","year":"2018","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-312135"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2016.210"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00563"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201600240"},{"key":"e_1_2_7_15_1","unstructured":"Probiotics in food: health and nutritional properties and guidelines for evaluation Food and Agriculture Organization of the United Nations: World Health Organization Rome2006.http:\/\/www.fao.org\/3\/a-a0512e.pdf"},{"key":"e_1_2_7_16_1","unstructured":"FAO\/WHO Joint FAO\/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria Cordoba Argentina2001.http:\/\/www.fao.org\/tempref\/docrep\/fao\/meeting\/009\/y6398e.pdf"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.07.003"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06983-11"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00243.2009"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201700144"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201490296"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01421-14"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.52.7.988"},{"key":"e_1_2_7_24_1","first-page":"137","volume":"6","author":"Gao X.","year":"2016","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3858"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.03.009"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.08.042"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5854285"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003887"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-091313-103739"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/291238a0"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2009.195"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00310"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3608"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2017.01.022"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neo.2016.04.001"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12748"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.04.001"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.223396"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01983"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2013.08.054"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01270"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2013.11.008"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01187-15"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2016.132"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf7419"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.03.007"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2011.09.006"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201540188"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20167324"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065718"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.4240\/wjgs.v8.i10.660"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055620"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2014.09.042"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmnfr.201800552","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.201800552","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T01:59:23Z","timestamp":1694743163000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.201800552"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,21]]},"references-count":53,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1002\/mnfr.201800552"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.201800552","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,21]]},"assertion":[{"value":"2018-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1800552"}}