{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T04:32:32Z","timestamp":1777437152086,"version":"3.51.4"},"reference-count":22,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2018,11,2]],"date-time":"2018-11-02T00:00:00Z","timestamp":1541116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009410","name":"Universitat de Lleida","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:sec>\n                    <jats:title>Scope<\/jats:title>\n                    <jats:p>The application of dried blood spot (DBS) cards for the study in human blood of dietary polyphenol bioavailability has been poorly studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>An analytical method based on blood sampling with DBS cards combined with LC\u2013MS\/MS has been developed and validated. To test the method validation, the phenolic metabolites are determined in human blood and plasma obtained after an acute intake of a red\u2010fleshed apple snack in ten volunteers. Capillary blood by finger prick is compared to venous blood by venipuncture and whole blood is also compared to their corresponding venous plasma samples. Moreover, the venous plasma results using DBS cards are compared to those obtained by microElution solid phase extraction (\u00b5SPE). The main phenolic metabolites detected in blood and plasma samples are phloretin glucuronide, dihydroxyphenylpropionic acid sulphate, (methyl) catechol sulphate, catechol glucuronide, and hydroxyphenyl\u2010\u03b3\u2010valerolactone glucuronide. No significant differences are observed between capillary blood, venous blood, and plasma samples using DBS, and neither between plasma samples analyzed by DBS or \u00b5SPE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Finger\u2010prick blood sampling based on DBS appears to be a suitable alternative to the classic invasive venipuncture for the determination of circulating phenolic metabolites in nutritional postprandial studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mnfr.201800623","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T06:14:03Z","timestamp":1539756843000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Validation of Dried Blood Spot Cards to Determine Apple Phenolic Metabolites in Human Blood and Plasma After an Acute Intake of Red\u2010Fleshed Apple Snack"],"prefix":"10.1002","volume":"62","author":[{"given":"Silvia","family":"Yuste","sequence":"first","affiliation":[{"name":"Food Technology Department Agrotecnio Research Center University of Lleida Av\/ Alcalde Rovira Roure 191 25198 Lleida Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alba","family":"Maci\u00e0","sequence":"additional","affiliation":[{"name":"Food Technology Department Agrotecnio Research Center University of Lleida Av\/ Alcalde Rovira Roure 191 25198 Lleida Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iziar A.","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Food Technology Department Agrotecnio Research Center University of Lleida Av\/ Alcalde Rovira Roure 191 25198 Lleida Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda\u2010Paz","family":"Romero","sequence":"additional","affiliation":[{"name":"Food Technology Department Agrotecnio Research Center University of Lleida Av\/ Alcalde Rovira Roure 191 25198 Lleida Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Fern\u00e1ndez\u2010Castillejo","sequence":"additional","affiliation":[{"name":"Universitat Rovira i Virgili Facultat de Medicina i Ci\u00e8ncies de la Salut Functional Nutrition, Oxidation, and Cardiovascular Diseases Group (NFOC\u2010Salut) Reus Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00darsula","family":"Catal\u00e1n","sequence":"additional","affiliation":[{"name":"Universitat Rovira i Virgili Facultat de Medicina i Ci\u00e8ncies de la Salut Functional Nutrition, Oxidation, and Cardiovascular Diseases Group (NFOC\u2010Salut) Reus Spain"},{"name":"Institut d'Investigaci\u00f3 Sanitaria Pere Virgili (IISPV) Reus Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda\u2010Jos\u00e9","family":"Motilva","sequence":"additional","affiliation":[{"name":"Food Technology Department Agrotecnio Research Center University of Lleida Av\/ Alcalde Rovira Roure 191 25198 Lleida Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Rubi\u00f3","sequence":"additional","affiliation":[{"name":"Food Technology Department Agrotecnio Research Center University of Lleida Av\/ Alcalde Rovira Roure 191 25198 Lleida Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,2]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21441"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-014-9695-3"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0031-6997(24)01329-2","volume":"49","author":"Hinderling P.H.","year":"1997","journal-title":"Pharmacol. 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