{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T06:16:54Z","timestamp":1789885014876,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T00:00:00Z","timestamp":1544572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Epigenetic aberrations caused by environmental factors and lifestyle choices have been associated with the development of a number of pathologies, including cardiovascular disorders. However, whether obesity\u2010related heart dysfunction can occur via epigenetic mechanisms is largely undisclosed. The manifested role of DNA hydroxymethylation in heart pathophysiology prompts an investigation of its levels\/machinery in heart of mice fed with high\u2010fat diet (HFD) and its possible relation with genes linked to obesity\u2010associated cardiac remodeling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Alterations in levels of DNA methylation\/hydroxymethylation modifications and in expression of Tet family of DNA hydroxylases are observed in hearts of mice treated with HFD for 8 and 16 weeks. Decreased levels of the Tet co\u2010substrate \u03b1\u2010ketoglutarate are also observed and associate with mitochondrial mass reduction and augmented oxidative stress. Finally, expression markers of cardiac remodeling are monitored by RT\u2010qPCR analysis and associate with DNA hydroxymethylation signature by DNA immunoprecipitation and correlation analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Global changes of DNA hydroxymethylation in hearts of HFD\u2010fed mice are associated with upregulation of the dioxygenase Tet3 and decreased content of \u03b1\u2010ketoglutarate. A relation between <jats:italic>Tet<\/jats:italic> genes and markers of cardiac hypertrophic response is observed and, if further validated, it will provide insights concerning epigenetics and obesity\u2010related cardiac complications.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201800970","type":"journal-article","created":{"date-parts":[[2018,12,5]],"date-time":"2018-12-05T06:50:38Z","timestamp":1543992638000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["High Dietary Fat Intake Affects DNA Methylation\/Hydroxymethylation in Mouse Heart: Epigenetic Hints for Obesity\u2010Related Cardiac Dysfunction"],"prefix":"10.1002","volume":"63","author":[{"given":"Fabio","family":"Ciccarone","sequence":"first","affiliation":[{"name":"Department of Biology University of Rome \u201cTor Vergata\u201d  00133 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Castelli","sequence":"additional","affiliation":[{"name":"Department of Biology University of Rome \u201cTor Vergata\u201d  00133 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ioannilli","sequence":"additional","affiliation":[{"name":"Department of Biology University of Rome \u201cTor Vergata\u201d  00133 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Rosa","family":"Ciriolo","sequence":"additional","affiliation":[{"name":"Department of Biology University of Rome \u201cTor Vergata\u201d  00133 Rome Italy"},{"name":"IRCCS San Raffaele \u2018La Pisana\u2019  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2017.10"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2017.33"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2016.11.038"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.04.001"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2012-13-8-r69"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14482"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu7020922"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2016.10.535"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature19081"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2017.06.008"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101022"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glx198"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.276568.115"},{"key":"e_1_2_7_11_3","doi-asserted-by":"publisher","DOI":"10.1530\/EC-17-0199"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12418"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02762-z"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0166575"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.311597"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcad.2018.07.012"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c170011"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2014.458"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0345-z"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.126417.111"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8768"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4875"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100832"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100579"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1179\/174329210X12650506623122"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00058.2015"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2010.12.001"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)80027-5"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21029"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.308999"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep43261"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.12.009"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.311300"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1210597"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-1001-5"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1848"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2013-14-10-r119"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvt264"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2018.05.012"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0b013e328364fb58"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40292-014-0068-x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.7.3467"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.2337\/db16-0387"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00664-8"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmnfr.201800970","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.201800970","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mnfr.201800970","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.201800970","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T21:18:14Z","timestamp":1694726294000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.201800970"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,12]]},"references-count":47,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1002\/mnfr.201800970"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.201800970","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,12]]},"assertion":[{"value":"2018-09-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1800970"}}