{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T16:34:37Z","timestamp":1788798877473,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2019,9,15]],"date-time":"2019-09-15T00:00:00Z","timestamp":1568505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2017R1A6A3A04011473"],"award-info":[{"award-number":["2017R1A6A3A04011473"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2019R1A2C1003218"],"award-info":[{"award-number":["2019R1A2C1003218"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Podocytes are a component of glomerular filtration barrier with interdigitating foot processes. The podocyte function depends on the dynamics of actin cytoskeletal and focal adhesion crucial for foot process structure. This study investigates the renoprotective effects of eucalyptol on the F\u2010actin cytoskeleton formation and focal adhesion assembly in glucose\u2010loaded podocytes and diabetic kidneys.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Eucalyptol at 1\u201320\u00a0\u00b5<jats:sc>m<\/jats:sc> reverses the reduction of cellular level of F\u2010actin, ezrin, cortactin, and Arp2\/3 in 33\u00a0m<jats:sc>m<\/jats:sc> glucose\u2010loaded mouse podocytes, and oral administration of 10\u00a0mg\u00a0kg<jats:sup>\u20131<\/jats:sup> eucalyptol elevates tissue levels of actin cytoskeletal proteins reduced in db\/db mouse kidneys. Eucalyptol inhibits podocyte morphological changes, showing F\u2010actin cytoskeleton formation in cortical regions and agminated F\u2010actin along the cell periphery. Eucalyptol induces focal adhesion proteins of paxillin, vinculin, talin1, FAK, and Src in glucose\u2010exposed podocytes and diabetic kidneys. Additionally, GTP\u2010binding Rac1, Cdc42, Rho A, and ROCK are upregulated in glucose\u2010stimulated podocytes and diabetic kidneys, which is attenuated by supplying eucalyptol. Rho A gene depletion partially diminishes GSK3\u03b2 induction of podocytes by glucose.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Eucalyptol ameliorates F\u2010actin cytoskeleton formation and focal adhesion assembly through blockade of the Rho signaling pathway, entailing partial involvement of GSK3\u03b2, which may inhibit barrier dysfunction of podocytes and resultant proteinuria.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201900489","type":"journal-article","created":{"date-parts":[[2019,9,4]],"date-time":"2019-09-04T19:09:53Z","timestamp":1567624193000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Eucalyptol Ameliorates Dysfunction of Actin Cytoskeleton Formation and Focal Adhesion Assembly in Glucose\u2010Loaded Podocytes and Diabetic Kidney"],"prefix":"10.1002","volume":"63","author":[{"given":"Dong Yeon","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Food and Nutrition Hallym University  Chuncheon 24252 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Kyung","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition Hallym University  Chuncheon 24252 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition Hallym University  Chuncheon 24252 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun\u2010Jung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition Hallym University  Chuncheon 24252 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeongjoo","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition Hallym University  Chuncheon 24252 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo\u2010Il","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition Hallym University  Chuncheon 24252 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su Yeon","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition Hallym University  Chuncheon 24252 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2039-7968","authenticated-orcid":false,"given":"Young\u2010Hee","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition Hallym University  Chuncheon 24252 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,15]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/35060051"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-003-0259-6"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2016.127"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2017.12.028"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5671803"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.113.21.3685"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-020911-153238"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009090991"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2017091039"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.04.007"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2337\/db08-1536"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000488231"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.55.01.06.db05-0894"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089233"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/2615286"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2014.06.027"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00394"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2013.09.003"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-015-1301-3"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-011-0274-4"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2017.30"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfv382"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201800302"},{"key":"e_1_2_7_24_1","doi-asserted-by":"crossref","first-page":"106190","DOI":"10.18632\/oncotarget.22311","volume":"8","author":"Kim D. 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