{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T14:04:12Z","timestamp":1788185052612,"version":"build-2803163510"},"reference-count":53,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T00:00:00Z","timestamp":1573171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31671803"],"award-info":[{"award-number":["31671803"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Val\u2010Ser\u2010Glu\u2010Glu (VSEE), identified from duck egg white peptides, has been proven to facilitate calcium absorption in a previous study. Since prevention of osteoporosis is important, it might act as a potential cofactor in osteoporosis prevention. Therefore, the aim of this study is to investigate the regulation of VSEE on osteoporosis and abnormal lipid metabolisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>MC3T3\u2010E1 cell and ovariectomized (OVX) rat model are used to evaluate VSEE on regulation of bone and lipid metabolisms. Differentiation and matrix mineralization of preosteoblast are significantly increased by VSEE (<jats:italic>p<\/jats:italic> &lt;0.05), which attributed to stimulating calcium influx, then to activating Wnt\/\u03b2\u2010catenin signaling pathway and regulating runt\u2010related transcription factor 2 and osteoprotegerin. VSEE can cross Caco\u20102\/HT\u201029 co\u2010cultured monolayer via paracellular pathway and peptide transporter 1 (PepT1), and can be detected in blood and maximum concentration is 122.84 \u00b1 3.68 mg L<jats:sup>\u22121<\/jats:sup> at 60 min. Additionally, VSEE reverses bone loss and regulate dyslipidemia through Wnt\/\u03b2\u2010catenin signaling pathway in OVX rats. Firmicutes phylum, Veillonellaceae, Prevotellaceae and six genera in VSEE group are significantly different compared with the Model group (<jats:italic>p<\/jats:italic> &lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>VSEE promotes bone growth and inhibit abnormal lipid metabolism in an OVX model through the regulation of intestinal microbiota compositions and Wnt\/\u03b2\u2010catenin signal pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201900525","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T16:35:56Z","timestamp":1572539756000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Duck Egg White\u2013Derived Peptide VSEE (Val\u2010Ser\u2010Glu\u2010Glu) Regulates Bone and Lipid Metabolisms by Wnt\/\u03b2\u2010Catenin Signaling Pathway and Intestinal Microbiota"],"prefix":"10.1002","volume":"63","author":[{"given":"Danjun","family":"Guo","sequence":"first","affiliation":[{"name":"College of Food Science and Technology Huazhong Agricultural University  Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Huazhong Agricultural University  Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Huazhong Agricultural University  Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengge","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Huazhong Agricultural University  Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biyang","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Huazhong Agricultural University  Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7567-2800","authenticated-orcid":false,"given":"Tao","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Huazhong Agricultural University  Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"He","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Huazhong Agricultural University  Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,8]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2009.260"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.061113"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.2001.5.4.205"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21388"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(17)30188-2"},{"key":"e_1_2_9_6_1","first-page":"S1094","author":"Danford C. 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