{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:31:59Z","timestamp":1790836319843,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T00:00:00Z","timestamp":1565913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p><jats:italic>Akkermansia muciniphila (A. muciniphila)<\/jats:italic> is an intestinal commensal with anti\u2010inflammatory properties both in the intestine and other organs. The aim is to investigate the effects of oral administration of <jats:italic>A. muciniphila<\/jats:italic> on lipid metabolism, immunity, and cuff\u2010induced neointima formation in hyperlipidemic APOE*3\u2010Leiden (E3L).CETP mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Hyperlipidemic male E3L.CETP mice are daily treated with 2\u00a0\u00d7\u00a010<jats:sup>8<\/jats:sup> CFU <jats:italic>A. muciniphila<\/jats:italic> by oral gavage for 4 weeks and the effects are determined on plasma lipid levels, immune parameters, and cuff\u2010induced neointima formation and composition. <jats:italic>A. muciniphila<\/jats:italic> administration lowers body weight and plasma total cholesterol and triglycerides levels. <jats:italic>A. muciniphila<\/jats:italic> influences the immune cell composition in mesenteric lymph nodes, as evident from an increased total B cell population, while reducing the total T cell and neutrophil populations. Importantly, <jats:italic>A. muciniphila<\/jats:italic> reduces the expression of the activation markers MHCII on dendritic cells and CD86 on B cells. <jats:italic>A. muciniphila<\/jats:italic> also increases whole blood ex vivo lipopolysaccharide\u2010stimulated IL\u201010 release. Finally, although treatment with <jats:italic>A. muciniphila<\/jats:italic> improves lipid metabolism and immunity, it does not affect neointima formation or composition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Four weeks of treatment with <jats:italic>A. muciniphila<\/jats:italic> exerts lipid\u2010lowering and immunomodulatory effects, which are insufficient to inhibit neointima formation in hyperlipidemic E3L.CETP mice.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201900732","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T06:55:07Z","timestamp":1565160907000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["<i>Akkermansia muciniphila<\/i> Exerts Lipid\u2010Lowering and Immunomodulatory Effects without Affecting Neointima Formation in Hyperlipidemic APOE*3\u2010Leiden.CETP Mice"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0993-9615","authenticated-orcid":false,"given":"Saeed","family":"Katiraei","sequence":"first","affiliation":[{"name":"Department of Human Genetics Leiden University Medical Center  2300 RC Leiden The Netherlands"},{"name":"Einthoven Laboratory for Experimental Vascular Medicine Leiden University Medical Center  2333 ZA Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margreet R.","family":"de Vries","sequence":"additional","affiliation":[{"name":"Einthoven Laboratory for Experimental Vascular Medicine Leiden University Medical Center  2333 ZA Leiden The Netherlands"},{"name":"Department of Surgery Leiden University Medical Center  2333 ZA Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice H.","family":"Costain","sequence":"additional","affiliation":[{"name":"Department of Parasitology Leiden University Medical Center  2333 ZA Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Thiem","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Radboud UMC  6525 GA Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa R.","family":"Hoving","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Leiden University Medical Center  2300 RC Leiden The Netherlands"},{"name":"Einthoven Laboratory for Experimental Vascular Medicine Leiden University Medical Center  2333 ZA Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janna A.","family":"van Diepen","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Radboud UMC  6525 GA Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermelijn H.","family":"Smits","sequence":"additional","affiliation":[{"name":"Department of Parasitology Leiden University Medical Center  2333 ZA Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristien E.","family":"Bouter","sequence":"additional","affiliation":[{"name":"Department of Vascular Medicine Academic Medical Center  1105 AZ Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick C. N.","family":"Rensen","sequence":"additional","affiliation":[{"name":"Einthoven Laboratory for Experimental Vascular Medicine Leiden University Medical Center  2333 ZA Leiden The Netherlands"},{"name":"Department of Medicine division of Endocrinology Leiden University Medical Center  2333 ZA Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul H. A.","family":"Quax","sequence":"additional","affiliation":[{"name":"Einthoven Laboratory for Experimental Vascular Medicine Leiden University Medical Center  2333 ZA Leiden The Netherlands"},{"name":"Department of Surgery Leiden University Medical Center  2333 ZA Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Nieuwdorp","sequence":"additional","affiliation":[{"name":"Department of Vascular Medicine Academic Medical Center  1105 AZ Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihai G.","family":"Netea","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Radboud UMC  6525 GA Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem M.","family":"de Vos","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Wageningen University  6708 WE Wageningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice D.","family":"Cani","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain Louvain Drug Research Institute WELBIO (Walloon Excellence in Life sciences and BIOtechnology) Metabolism and Nutrition Research Group  1200 Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Belzer","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Wageningen University  6708 WE Wageningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ko Willems","family":"van Dijk","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Leiden University Medical Center  2300 RC Leiden The Netherlands"},{"name":"Einthoven Laboratory for Experimental Vascular Medicine Leiden University Medical Center  2333 ZA Leiden The Netherlands"},{"name":"Department of Medicine division of Endocrinology Leiden University Medical Center  2333 ZA Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimmy F. 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