{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:59:52Z","timestamp":1790139592850,"version":"4.0.1"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Insects are a potentially environmentally friendly alternative dietary protein source to supplement mammalian and fish sources, but potential allergenic risks are a concern. Consumption of insects may result in anaphylaxis and has been implicated in cross\u2010reactivity with shellfish. Many allergenic proteins may be involved in cross\u2010reactivity, including tropomyosin (TM). The uniformity of TM cross\u2010reactivity among edible insects is unknown. Candidate edible insects for variability in shellfish IgE cross\u2010reactivity are investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Selected insects and known related sources of allergens are extracted and probed by immunoblot with sera\/plasma from patients sensitized to insects or shellfish. Quantification of TM in these extracts is performed using mass spectrometry. A comparison of the quantity of TM and the IgE reactivity of TM from these insects is performed. Distinct patterns of IgE cross\u2010reactivity are observed with three insect species showing diminished reactivity. This pattern is not consistent with the amount of TM present in these insects, or with overall sequence homology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Insects display a diversity of TM\u2010associated IgE reactivity. It is likely that minor sequence features and\/or structural effects are primarily responsible. Additionally, it is demonstrated that some insect species may present significantly less IgE cross\u2010reactivity to shrimp than do others.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201900923","type":"journal-article","created":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T09:53:54Z","timestamp":1582019634000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Shellfish Tropomyosin IgE Cross\u2010Reactivity Differs Among Edible Insect Species"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7532-2341","authenticated-orcid":false,"given":"Lee K.","family":"Palmer","sequence":"first","affiliation":[{"name":"University of Nebraska\u2010Lincoln  1901 N 21<sup>st<\/sup> St. Lincoln NE 68503 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin T.","family":"Marsh","sequence":"additional","affiliation":[{"name":"University of Nebraska\u2010Lincoln  1901 N 21<sup>st<\/sup> St. Lincoln NE 68503 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Lu","sequence":"additional","affiliation":[{"name":"University of Nebraska\u2010Lincoln  1901 N 21<sup>st<\/sup> St. Lincoln NE 68503 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard E.","family":"Goodman","sequence":"additional","affiliation":[{"name":"University of Nebraska\u2010Lincoln  1901 N 21<sup>st<\/sup> St. Lincoln NE 68503 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Zeece","sequence":"additional","affiliation":[{"name":"University of Nebraska\u2010Lincoln  1901 N 21<sup>st<\/sup> St. Lincoln NE 68503 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip E.","family":"Johnson","sequence":"additional","affiliation":[{"name":"University of Nebraska\u2010Lincoln  1901 N 21<sup>st<\/sup> St. Lincoln NE 68503 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-120811-153704"},{"key":"e_1_2_8_2_1","volume-title":"Edible Insects: Future Prospects for Food and Feed Security","author":"Huis A.","year":"2013"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/10903127.2016.1204035"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1665\/034.023.0105"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.01.035"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201601061"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2016.07.015"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201700030"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.04.008"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2015.10.033"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.04.038"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E14-12-1619"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000065172"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.7b06042"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.08.019"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.108434"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2015.05.013"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/756789"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0715471"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(82)85023-4"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.016345"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.03.015"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2016.e00218"},{"key":"e_1_2_8_20_1","first-page":"955","volume":"37","author":"Choi B. 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