{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T15:25:25Z","timestamp":1778599525694,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T00:00:00Z","timestamp":1571788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["691042"],"award-info":[{"award-number":["691042"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>As a result of the obesity epidemic, the prevalence of non\u2010alcoholic steatohepatitis (NASH) is increasing. No drug is approved for the treatment of NASH. In this study, the effect of a nutritional supplement, Mastiha or Chios mastic gum, on metabolic and histological parameters and on the gut microbiome in mice with NASH and fibrosis was investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Advanced NASH was induced by feeding C57BL\/6J mice a diet rich in fat, sucrose, and cholesterol for 41 weeks. After randomization, animals received the NASH\u2010inducing diet with or without 0.2% (w\/w) Mastiha for a further 8 weeks. Disease activity was assessed by liver histology and determination of plasma transaminase activities. Fecal microbiota DNA extraction and 16S rRNA amplicon sequencing were used to determine the composition of the gut microbiome.<\/jats:p><jats:p>Mastiha supplementation led to a significant reduction in circulating alanine aminotransferase (ALT) activity, improvement in hepatic steatosis and collagen content, and a reduction in NAFLD activity score. Furthermore, it resulted in a partial but significant recovery of gut microbiota diversity and changes in identity and abundance of specific taxa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is the first study demonstrating an improvement in disease activity in mice with advanced NASH with fibrosis by a diet containing Mastiha.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201900927","type":"journal-article","created":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T07:10:51Z","timestamp":1570691451000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Mastiha (<i>Pistacia lentiscus<\/i>) Improves Gut Microbiota Diversity, Hepatic Steatosis, and Disease Activity in a Biopsy\u2010Confirmed Mouse Model of Advanced Non\u2010Alcoholic Steatohepatitis and Fibrosis"],"prefix":"10.1002","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5197-2286","authenticated-orcid":false,"given":"Aimo","family":"Kannt","sequence":"first","affiliation":[{"name":"Sanofi Research and Development  65926 Frankfurt Germany"},{"name":"Institute of Experimental Pharmacology, Medical Faculty Mannheim University of Heidelberg  68167 Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Efstathia","family":"Papada","sequence":"additional","affiliation":[{"name":"Harokopio University Athens  Kallithea 17676 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Kammermeier","sequence":"additional","affiliation":[{"name":"Sanofi Research and Development  65926 Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"D'Auria","sequence":"additional","affiliation":[{"name":"Foundation for the Promotion of Health and Biomedical Research (FISABIO)  46035 Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuria","family":"Jim\u00e9nez\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Foundation for the Promotion of Health and Biomedical Research (FISABIO)  46035 Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Stephan","sequence":"additional","affiliation":[{"name":"Sanofi Research and Development  65926 Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe","family":"Schwahn","sequence":"additional","affiliation":[{"name":"Sanofi Research and Development  65926 Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas Nygaard","family":"Madsen","sequence":"additional","affiliation":[{"name":"Gubra  2970 Horsholm Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mette Viberg","family":"\u00d8stergaard","sequence":"additional","affiliation":[{"name":"Gubra  2970 Horsholm Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Dedoussis","sequence":"additional","affiliation":[{"name":"Harokopio University Athens  Kallithea 17676 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Pilar","family":"Francino","sequence":"additional","affiliation":[{"name":"Foundation for the Promotion of Health and Biomedical Research (FISABIO)  46035 Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"for the MAST4HEALTH consortium","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,10,23]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29085"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.109"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28431"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2017.09.021"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0047-9"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.04.005"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.04.014"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)00803-X"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2015.12.012"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-018-5080-x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0907929"},{"key":"e_1_2_7_12_1","unstructured":"European Medicines Agency. European Union herbal monograph on Pistacia lentiscus L. resin (mastix) 2015. Available at:https:\/\/www.ema.europa.eu\/en\/documents\/herbal-monograph\/draft-european-union-herbal-monograph-pistacia-lentiscus-l-resin-mastix_en.pdf(accessed: September 2019)."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.454"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00642-06"},{"key":"e_1_2_7_15_1","first-page":"354","volume":"71","author":"Heo C.","year":"2006","journal-title":"Korean J. Med."},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2004.02.011"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2006.03.009"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/2047487315603186"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.13.6031"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i5.748"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2013.0069"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v8.i16.673"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00079.2013"},{"key":"e_1_2_7_24_1","unstructured":"US Food and Drug Administration Final Determination Regarding Partially Hydrogenated Oils (Removing Trans Fat).2018; Available at:https:\/\/www.federalregister.gov\/documents\/2018\/05\/21\/2018-10714\/final-determination-regarding-partially-hydrogenated-oils(accessed: September 2019)."},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v25.i33.4904"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-0105.177703"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8070208"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20701"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks808"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr026"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1219"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.13643"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2009.09.010"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13668-017-0190-1"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6941.12228"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.08.042"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.112"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201700751"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmnfr.201900927","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.201900927","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mnfr.201900927","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.201900927","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T17:22:47Z","timestamp":1694193767000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.201900927"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,23]]},"references-count":40,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1002\/mnfr.201900927"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.201900927","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,23]]},"assertion":[{"value":"2019-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1900927"}}