{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T05:33:03Z","timestamp":1763443983182,"version":"3.41.2"},"reference-count":59,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T00:00:00Z","timestamp":1586736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI11\/00357","PI15\/01686","PIE16\/00050","CD17\/00203"],"award-info":[{"award-number":["PI11\/00357","PI15\/01686","PIE16\/00050","CD17\/00203"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Postprandial dysmetabolism plays a major role in the pathogenesis of metabolic disorders such as obesity and the polycystic ovary syndrome (PCOS). The aim is to characterize the circulating lipoprotein particle profiles in response to oral glucose, lipid, and protein challenges.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>17 women with PCOS, 17 control women, and 19 healthy men selected to have similar age and body mass index are studied. Blood samples are collected following the ingestion of 300\u00a0kcal in the form of glucose, lipids, or proteins, and they are submitted to two\u2010dimensional (2D) diffusion\u2010ordered <jats:sup>1<\/jats:sup>H\u2010NMR spectroscopy. Regardless of macronutrient administered, the number of very low\u2010density (VLDL) particles increases whereas low density\u2010lipoprotein (LDL) decreases. High density\u2010lipoprotein (HDL) particles increase only after lipid ingestion. Obese subjects show an increase in the number of large VLDL particles and a decrease in large LDL particles, with a significant reduction in the average particle size of LDL. Patients with PCOS show a particularly unfavorably smaller LDL particle size response to oral lipid intake, regardless of obesity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Oral macronutrient challenges induce immediate class\u2010specific postprandial changes in particle number and size of lipoproteins, with lipids inducing a more pro\u2010atherogenic lipoprotein profile compared to glucose and proteins, particularly in obese subjects and women with PCOS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201900928","type":"journal-article","created":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T20:46:33Z","timestamp":1584564393000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["2D Diffusion\u2010Ordered <sup>1<\/sup>H\u2010NMR Spectroscopy Lipidomic Profiling after Oral Single Macronutrient Loads: Influence of Obesity, Sex, and Female Androgen Excess"],"prefix":"10.1002","volume":"64","author":[{"given":"Mar\u00eda","family":"Insenser","sequence":"first","affiliation":[{"name":"Diabetes, Obesity and Human Reproduction Research Group Department of Endocrinology &amp; Nutrition Hospital Universitario Ram\u00f3n y Cajal &amp; Universidad de Alcal\u00e1 &amp; Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS) &amp; Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM)  Madrid E\u201028034 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Moncayo","sequence":"additional","affiliation":[{"name":"Diabetes, Obesity and Human Reproduction Research Group Department of Endocrinology &amp; Nutrition Hospital Universitario Ram\u00f3n y Cajal &amp; Universidad de Alcal\u00e1 &amp; Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS) &amp; Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM)  Madrid E\u201028034 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. \u00c1ngeles","family":"Mart\u00ednez\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Diabetes, Obesity and Human Reproduction Research Group Department of Endocrinology &amp; Nutrition Hospital Universitario Ram\u00f3n y Cajal &amp; Universidad de Alcal\u00e1 &amp; Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS) &amp; Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM)  Madrid E\u201028034 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Fern\u00e1ndez\u2010Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Diabetes, Obesity and Human Reproduction Research Group Department of Endocrinology &amp; Nutrition Hospital Universitario Ram\u00f3n y Cajal &amp; Universidad de Alcal\u00e1 &amp; Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS) &amp; Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM)  Madrid E\u201028034 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Samino","sequence":"additional","affiliation":[{"name":"Departament d'Enginyeria Electr\u00f2nica El\u00e8ctrica i Autom\u00e0tica Universitat Rovira i Virgili  Reus Tarragona E\u201043007 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"\u00c1lvarez\u2010Blasco","sequence":"additional","affiliation":[{"name":"Diabetes, Obesity and Human Reproduction Research Group Department of Endocrinology &amp; Nutrition Hospital Universitario Ram\u00f3n y Cajal &amp; Universidad de Alcal\u00e1 &amp; Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS) &amp; Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM)  Madrid E\u201028034 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Luque\u2010Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Diabetes, Obesity and Human Reproduction Research Group Department of Endocrinology &amp; Nutrition Hospital Universitario Ram\u00f3n y Cajal &amp; Universidad de Alcal\u00e1 &amp; Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS) &amp; Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM)  Madrid E\u201028034 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6890-1644","authenticated-orcid":false,"given":"H\u00e9ctor F.","family":"Escobar\u2010Morreale","sequence":"additional","affiliation":[{"name":"Diabetes, Obesity and Human Reproduction Research Group Department of Endocrinology &amp; Nutrition Hospital Universitario Ram\u00f3n y Cajal &amp; Universidad de Alcal\u00e1 &amp; Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS) &amp; Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM)  Madrid E\u201028034 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,13]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2007.03.107"},{"key":"e_1_2_8_2_1","first-page":"168","volume":"28","author":"Higgins V.","year":"2017","journal-title":"EJIFCC"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deu198"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/oby.22073"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9136(199609)13:9<816::AID-DIA183>3.0.CO;2-L"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-68-3-647"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.48.8.1586"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2337\/db05-1692"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.110.136432"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-511X-10-181"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2006.07.003"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-511X-12-179"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-1786"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2018.24"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2011.579658"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2008.164"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.104.512079"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2011.03.013"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.17.7.1447"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2015-2566"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-2724"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.2011.04015.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.166.19.2081"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-3918"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/det057"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2015.07.040"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.2.453"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2016-1500"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2011.04.007"},{"key":"e_1_2_8_30_1","first-page":"E798","volume":"275","author":"Sidossis L. S.","year":"1998","journal-title":"Endocrinol. Metab."},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114155"},{"key":"e_1_2_8_32_1","first-page":"220","volume":"102","author":"R\u00e4m\u00f6 J. T.","year":"2017","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(91)90305-V"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.42.6.833"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/73.2.198"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(00)00424-X"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000131511.50734.44"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(95)90134-5"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.17.6.1082"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1067\/mhj.2002.120302"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1053\/meta.2001.20202"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2015.00218"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.565135"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.113.002671"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2012.03.060"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacl.2011.02.001"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201700425"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2019.03.027"},{"key":"e_1_2_8_49_1","first-page":"377","volume-title":"Polycystic Ovary Syndrome","author":"Zawadzki J. K.","year":"1992"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072890"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(00)00718-4"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.84.10.6079"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00280883"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.22.9.1462"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc18-S002"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D050120"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2006.07.006"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-2327"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10010111"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmnfr.201900928","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.201900928","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mnfr.201900928","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.201900928","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:36:52Z","timestamp":1694047012000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.201900928"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,13]]},"references-count":59,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1002\/mnfr.201900928"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.201900928","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"type":"print","value":"1613-4125"},{"type":"electronic","value":"1613-4133"}],"subject":[],"published":{"date-parts":[[2020,4,13]]},"assertion":[{"value":"2019-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1900928"}}