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Methionine\u2010 and choline\u2010deficient diets (MCDD) induce fatty liver in mice which is partly mediated by triglyceride (TG) lipolysis in white adipose tissues (WATs). Because Fatp4 knockdown has been shown to increase adipocyte lipolysis in vitro, here, the effects of MCDD on WAT lipolysis in <jats:italic>aP2<\/jats:italic>\u2010Cre Fatp4\u2010knockout (Fatp4<jats:sup>A\u2212\/\u2212<\/jats:sup>) mice are determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>Isolated WATs of Fatp4<jats:sup>A\u2212\/\u2212<\/jats:sup> mice exposed to MCD medium show an increase in lipolysis, and the strongest effect is noted on glycerol release from subcutaneous fat. Fatp4<jats:sup>A\u2212\/\u2212<\/jats:sup> mice fed with MCDD for 4 weeks show an increase in serum glycerol, TG, and leptin levels associated with the activation of hormone\u2010sensitive lipase in subcutaneous fat. Chow\u2010fed Fatp4<jats:sup>A\u2212\/\u2212<\/jats:sup> mice also show an increase in serum leptin and very\u2010low\u2010density lipoproteins as well as liver phosphatidylcholine and sphingomyelin levels. Both chow\u2010 and MCDD\u2010fed Fatp4<jats:sup>A\u2212\/\u2212<\/jats:sup> mice show a decrease in serum ketone and WAT sphingomyelin levels which supports a metabolic shift to TG for subsequent WAT lipolysis<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Adipose Fatp4 deficiency leads to TG lipolysis and leptin release, which are exaggerated by MCDD. The data imply hyperlipidemia risk by a low dietary choline intake and gene mutations that increase adipose TG levels.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202000361","type":"journal-article","created":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T21:59:59Z","timestamp":1601416799000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Methionine\u2010 and Choline\u2010Deficient Diet Enhances Adipose Lipolysis and Leptin Release in <i>aP2<\/i>\u2010Cre Fatp4\u2010Knockout Mice"],"prefix":"10.1002","volume":"64","author":[{"given":"Yuting","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Internal Medicine IV University of Heidelberg Hospital  Im Neuenheimer Feld 410 69120 Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongying","family":"Gan\u2010Schreier","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV University of Heidelberg Hospital  Im Neuenheimer Feld 410 69120 Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"See\u00dfle","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV University of Heidelberg Hospital  Im Neuenheimer Feld 410 69120 Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Staffer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV University of Heidelberg Hospital  Im Neuenheimer Feld 410 69120 Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Tuma\u2010Kellner","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV University of Heidelberg Hospital  Im Neuenheimer Feld 410 69120 Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Khnykin","sequence":"additional","affiliation":[{"name":"Department of Pathology and Center for Immune Regulation Rikshospitalet University Hospital  0424 Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Stremmel","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV University of Heidelberg Hospital  Im Neuenheimer Feld 410 69120 Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uta","family":"Merle","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV University of Heidelberg Hospital  Im Neuenheimer Feld 410 69120 Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Westkuesten Hospital  Esmarchstra\u00dfe 50 25746 Heide Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5999-7664","authenticated-orcid":false,"given":"Walee","family":"Chamulitrat","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine IV University of Heidelberg Hospital  Im Neuenheimer Feld 410 69120 Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,12]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2009.00246.x"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10010004"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/NT.0000000000000302"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/84.1.88"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0155403"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504285102"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127991"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M800042-JLR200"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2014.08.015"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2014.02.012"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10426"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108640200"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.1016\/S0022-2275(20)33310-1","volume":"39","author":"Abumrad N.","year":"1998","journal-title":"J. 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