{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:02:38Z","timestamp":1753869758215,"version":"3.41.2"},"reference-count":49,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2021,8,2]],"date-time":"2021-08-02T00:00:00Z","timestamp":1627862400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Perinatal nutritional factors can program offspring metabolic phenotype and risk to obesity. This study investigates the potential role of leptin supplementation (during lactation) in ameliorating the malprogrammed effects caused by mild maternal calorie restriction during gestation, on young rat offspring liver metabolic response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>Untargeted and targeted metabolomics studies on liver samples are performed by NMR and GC\u2010MS, respectively. Global DNA methylation and the expression by RT\u2010PCR of key genes involved in different pathways are also determined. By NMR, 15 liver metabolites are observed to be altered in the offspring of gestational calorie\u2010restricted dams (CR group), at days 25\u201327 of life. Physiological leptin supplementation during lactation partially reverted the effect of CR condition for most of these metabolites. Moreover, targeted fatty acid analysis by GC\u2010MS shows a significant decrease in the hepatic concentration of certain very long\u2010chain fatty acids (VLCFA) in CR offspring, partially or totally reverted by leptin supplementation. No remarkable changes are found in global DNA methylation or mRNA expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Physiological leptin supplementation during lactation contributes to the reversion of changes caused by maternal mild calorie restriction on the liver metabolome. This agrees with a putative role of leptin supplementation preventing or reversing metabolic disturbances caused by gestational metabolic malprogramming.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202001046","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T09:34:24Z","timestamp":1619429664000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Leptin Supplementation During Lactation Restores Key Liver Metabolite Levels Malprogrammed by Gestational Calorie Restriction"],"prefix":"10.1002","volume":"65","author":[{"given":"Zhi Xin","family":"Yau\u2010Qiu","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology Nutrition and Biotechnology (Nutrigenomics and obesity) University of the Balearic Islands (UIB)  Palma de Mallorca Spain"},{"name":"Health Research Institute of the Balearic Islands (IdISBa)  Palma de Mallorca Spain"},{"name":"CIBER Fisiopatolog\u00eda de la Obesidad y Nutrici\u00f3n (CIBEROBN)  Palma de Mallorca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Madrid\u2010Gambin","sequence":"additional","affiliation":[{"name":"UCD School of Agriculture and Food Science Institute of Food and Health Conway Institute University College Dublin (UCD)  Dublin Ireland"},{"name":"Department of Psychiatry Royal College of Surgeons in Ireland Beaumont Hospital  Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7711-7499","authenticated-orcid":false,"given":"Lorraine","family":"Brennan","sequence":"additional","affiliation":[{"name":"UCD School of Agriculture and Food Science Institute of Food and Health Conway Institute University College Dublin (UCD)  Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreu","family":"Palou","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology Nutrition and Biotechnology (Nutrigenomics and obesity) University of the Balearic Islands (UIB)  Palma de Mallorca Spain"},{"name":"Health Research Institute of the Balearic Islands (IdISBa)  Palma de Mallorca Spain"},{"name":"CIBER Fisiopatolog\u00eda de la Obesidad y Nutrici\u00f3n (CIBEROBN)  Palma de Mallorca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1559-6279","authenticated-orcid":false,"given":"Ana Mar\u00eda","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology Nutrition and Biotechnology (Nutrigenomics and obesity) University of the Balearic Islands (UIB)  Palma de Mallorca Spain"},{"name":"Health Research Institute of the Balearic Islands (IdISBa)  Palma de Mallorca Spain"},{"name":"CIBER Fisiopatolog\u00eda de la Obesidad y Nutrici\u00f3n (CIBEROBN)  Palma de Mallorca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,2]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(01)00721-3"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1375\/twin.4.5.287"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1326.2009.01174.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2011.11.005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2014.56"},{"key":"e_1_2_9_6_1","volume":"6","author":"Garc\u00eda A. 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