{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T23:02:49Z","timestamp":1783119769776,"version":"3.54.6"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,3,11]],"date-time":"2021-03-11T00:00:00Z","timestamp":1615420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009578","name":"Mizutani Foundation for Glycoscience","doi-asserted-by":"publisher","award":["#130097"],"award-info":[{"award-number":["#130097"]}],"id":[{"id":"10.13039\/100009578","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Lactoferrin (Lf), a sialylated milk glycoprotein, promotes early neurodevelopment and cognition. Functional concentrations of Lf, however, remain unknown. Our objective is to determine the concentration\u2010dependency of Lf on genes associated with neurodevelopment and cognition in neonatal piglets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Piglets are given milk replacer with Lf at concentrations of 155 (low) or 285 mg kg<jats:sup>\u20101<\/jats:sup> day<jats:sup>\u20101<\/jats:sup> (high) from postnatal days 3 to 38. Gene expression associated with neurodevelopment, cognition, and cognate proteins were quantitated. This study found 1) The rate of learning and long\u2010term memory was higher with 155 mg kg<jats:sup>\u20101<\/jats:sup> day<jats:sup>\u20101<\/jats:sup> assessed in an eight\u2010arm radial maze; 2) Global gene transcription profiling showed this lower concentration upregulated genes and functions correlated with neurodevelopment and cognition, while the higher concentration regulated cellular processes for neuroprotection; 3) Expression of BDNF genes and proteins were higher with both concentrations, while genes regulating BDNF signaling, including SLC6A3, IGF\u20101 responded more to the lower concentration; 4) The lower concentration modulated genes in the five highest networks associated with cellularity and neurocognition, while the prevention of neurodevelopmental and neurological pathologies was associated with the higher concentration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The lower concentrations of Lf enhanced neurodevelopment and cognition, while higher concentrations are greater neuroprotective, findings of potential novel clinical relevance.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202001099","type":"journal-article","created":{"date-parts":[[2021,2,28]],"date-time":"2021-02-28T08:29:50Z","timestamp":1614500990000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Functional Correlates and Impact of Dietary Lactoferrin Intervention and its Concentration\u2010dependence on Neurodevelopment and Cognition in Neonatal Piglets"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7909-4606","authenticated-orcid":false,"given":"Yue","family":"Chen","sequence":"first","affiliation":[{"name":"School of Medicine Xiamen University  Xiamen 361005 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2146-8529","authenticated-orcid":false,"given":"Bing","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine Xiamen University  Xiamen 361005 China"},{"name":"Graham Centre for Agricultural Innovation Charles Sturt University  NSW 2678 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changwei","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Medicine Xiamen University  Xiamen 361005 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Shi","sequence":"additional","affiliation":[{"name":"Nestle Research Centre  Beijing 100095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhizhong","family":"Dong","sequence":"additional","affiliation":[{"name":"Nutrition &amp; Health Research Institute COFCO Corporation  Beijing 102209 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"II","given":"Frederic A.","family":"Troy","sequence":"additional","affiliation":[{"name":"School of Medicine Xiamen University  Xiamen 361005 China"},{"name":"Department of Biochemistry and Molecular Medicine University of California School of Medicine  Davis CA 95616 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,11]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2016.05.013"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2016.02.073"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1037\/0033-2909.105.2.179"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.28.061807.155515"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3fo60298b"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2011.12.014"},{"key":"e_1_2_8_7_1","first-page":"252","volume":"80","author":"Levay P. 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