{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:49:07Z","timestamp":1792172947740,"version":"4.4.0"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T00:00:00Z","timestamp":1618531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["691042"],"award-info":[{"award-number":["691042"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease with poor therapeutic strategies. Mastiha possesses antioxidant\/anti\u2010inflammatory and lipid\u2010lowering properties. The authors investigate the effectiveness of Mastiha as a nonpharmacological intervention in NAFLD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>Ninety\u2010eight patients with NAFLD in three countries (Greece, Italy, Serbia) are randomly allocated to either Mastiha or Placebo for 6 months, as part of a multicenter, randomized, double\u2010blind, placebo\u2010controlled, parallel\u2010group clinical trial. The authors assess NAFLD severity via magnetic resonance imaging (MRI) scanning and LiverMultiScan technique and evaluate the effectiveness of Mastiha through medical, anthropometric, biochemical, metabolomic, and microbiota assessment. Mastiha is not superior to Placebo on changes in iron\u2010corrected T1 (cT1) and Liver Inflammation Fibrosis score (LIF) in entire patient population; however, after BMI stratification (BMI \u2264 35\u00a0kg m<jats:sup>\u20102<\/jats:sup> and BMI &gt; 35\u00a0kg m<jats:sup>\u20102<\/jats:sup>), severely obese patients show an improvement in cT1 and LIF in Mastiha versus Placebo. Mastiha increases dissimilarity of gut microbiota, as shown by the Bray\u2010Curtis index, downregulates <jats:italic>Flavonifractor<\/jats:italic>, a known inflammatory taxon and decreases Lysophosphatidylcholines\u2010(LysoPC) 18:1, Lysophosphatidylethanolamines\u2010(LysoPE) 18:1, and cholic acid compared to Placebo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Mastiha supplementation improves microbiota dysbiosis and lipid metabolite levels in patients with NAFLD, although it reduces parameters of liver inflammation\/fibrosis only in severely obese patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202001178","type":"journal-article","created":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T03:09:51Z","timestamp":1614222591000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Effect of Mastiha supplementation on NAFLD: The MAST4HEALTH Randomised, Controlled Trial"],"prefix":"10.1002","volume":"65","author":[{"given":"Charalampia","family":"Amerikanou","sequence":"first","affiliation":[{"name":"Department of Nutrition and Dietetics, School of Health Science and Education Harokopio University  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stavroula","family":"Kanoni","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Barts and The London School of Medicine and Dentistry Queen Mary University of London  London EC1M 6BQ UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andriana C.","family":"Kaliora","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, School of Health Science and Education Harokopio University  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Barone","sequence":"additional","affiliation":[{"name":"Perspectum Ltd  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mladen","family":"Bjelan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of Novi Sad  Novi Sad Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"D'Auria","sequence":"additional","affiliation":[{"name":"Sequencing and Bioinformatics Service Fundaci\u00f3 per al Foment de la Investigaci\u00f3 Sanit\u00e0ria i Biom\u00e8dica de la Comunitat Valenciana (FISABIO\u2010Salut P\u00fablica)  Avda. 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