{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:36:17Z","timestamp":1790742977964,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T00:00:00Z","timestamp":1622160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Vitamin B12 and folate (methyl donors) deficiency is frequent during pregnancy. Experimental rat models with methyl donor deficit during pregnancy and lactation (Initial methyl donor deficit (iMDD)) produce impaired myocardium fatty acid oxidation and mitochondrial energy metabolism at weaning.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>The consequences of iMDD on heart of rat pups under normal diet after weaning and high fat diet (HF) between day (D) 50 and D185 are investigated. iMDD\/HF induces increased histological fibrosis and increased B\u2010type natriuretic peptide blood level. Inflammation is evidenced by increased protein expression of NFkB, Caspase1, and IL1\u03b2 and fibrosis by increased expression of \u03b1SMA, col1a1, and col1a2 in females, but not in males. Fibrosis is related to increased angiotensin at D50 and D185 and increased protein expression of TGFB1 and AT1 angiotensin receptors at D185. The limited fibrosis in males is consistent with increased expression of AT2, the antagonist receptor of AT1. The increased expression of GLUT4 and decreased expression of PGC1\u03b1 and PPAR\u03b1 reflect a shift from fatty acid oxidation to glycolysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Developmental programming by iMDD produces cardiomyopathy in female offspring exposed to HF. The cardiomyopathy is linked to inflammation and fibrosis through angiotensin\u2010AT2 and TGFB1 pathways and alteration of energy metabolism.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202100065","type":"journal-article","created":{"date-parts":[[2021,5,15]],"date-time":"2021-05-15T14:46:21Z","timestamp":1621089981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Programming by Methyl Donor Deficiency during Pregnancy and Lactation Produces Cardiomyopathy in Adult Rats Subjected to High Fat Diet"],"prefix":"10.1002","volume":"65","author":[{"given":"Zhen","family":"Li","sequence":"first","affiliation":[{"name":"UMR Inserm 1256 N\u2010GERE (Nutrition, G\u00e9netique et Exposition aux Risques Environmentaux) Universit\u00e9 de Lorraine  Vandoeuvre\u2010l\u00e8s\u2010Nancy 54500 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viola 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