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The potential of nicotinamide (NAM), the amide form of vitamin B3 and a physiological precursor of nicotinamide adenine dinucleotide (NAD)<jats:sup>+<\/jats:sup>, in preventing weight gain has not previously been studied in vivo. Other NAD<jats:sup>+<\/jats:sup> precursors have been shown to decrease weight gain; however, their impact on adipose tissue is not addressed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Two doses of NAM (high dose: 1% and low dose: 0.25%) are given by drinking water to C57BL\/6J male mice, starting at the same time as the high\u2010fat diet feeding. NAM supplementation protects against diet\u2010induced obesity by augmenting global body energy expenditure in C57BL\/6J male mice. The manipulation markedly alters adipose morphology and metabolism, particularly in inguinal (i) white adipose tissue (iWAT). An increased number of brown and beige adipocyte clusters, protein abundance of uncoupling protein 1 (UCP1), mitochondrial activity, adipose NAD<jats:sup>+<\/jats:sup>, and phosphorylated AMP\u2010activated protein kinase (P\u2010AMPK) levels are observed in the iWAT of treated mice. Notably, a significant improvement in hepatic steatosis, inflammation, and glucose tolerance is also observed in NAM high\u2010dose treated mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>NAM influences whole\u2010body energy expenditure by driving changes in the adipose phenotype. Thus, NAM is an attractive potential treatment for preventing obesity and associated complications.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202100111","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T05:20:23Z","timestamp":1618809623000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Nicotinamide Protects Against Diet\u2010Induced Body Weight Gain, Increases Energy Expenditure, and Induces White Adipose Tissue Beiging"],"prefix":"10.1002","volume":"65","author":[{"given":"Karen Alejandra","family":"M\u00e9ndez\u2010Lara","sequence":"first","affiliation":[{"name":"Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau i Institut d'Investigaci\u00f3 Biom\u00e8dica de l'Hospital de la Santa Creu i Sant Pau IIB\u2010Sant Pau  Barcelona 08041 Spain"},{"name":"Departament de Bioqu\u00edmica i Biologia Molecular Universitat Aut\u00f2noma de Barcelona (UAB)  Barcelona 08193 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Rodr\u00edguez\u2010Mill\u00e1n","sequence":"additional","affiliation":[{"name":"Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau i Institut d'Investigaci\u00f3 Biom\u00e8dica de l'Hospital de la Santa Creu i Sant Pau IIB\u2010Sant Pau  Barcelona 08041 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Sebasti\u00e1n","sequence":"additional","affiliation":[{"name":"Departament de Bioqu\u00edmica i Biomedicina Facultat de Biologia Universitat de Barcelona (UB)  Barcelona 08028 Spain"},{"name":"Institute for Research in Biomedicine (IRB Barcelona) The Barcelona Institute of Science and Technology  Barcelona 08028 Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas CIBERDEM  Madrid 28028 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosi","family":"Blanco\u2010Soto","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Bioingenier\u00eda Biomateriales y Nanomedicina CIBER\u2010BBN  Madrid 28028 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5970-3294","authenticated-orcid":false,"given":"Mercedes","family":"Camacho","sequence":"additional","affiliation":[{"name":"Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau i Institut d'Investigaci\u00f3 Biom\u00e8dica de l'Hospital de la Santa Creu i Sant Pau IIB\u2010Sant Pau  Barcelona 08041 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madalina N.","family":"Nan","sequence":"additional","affiliation":[{"name":"Servei de Bioqu\u00edmica Hospital de la Santa Creu i Sant Pau  IIB\u2010Sant Pau Barcelona 08041 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena M. 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