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Compared to the wild type littermates (WT), the <jats:italic>dp\/+<\/jats:italic> mice exhibit obvious obese phenotype characterized by significant increase in body mass index, fat pad mass, and fat ratio, with visceral\u2010dominant fat deposits at 12\u2010week age. White adipose tissue (WAT), liver tissue, and plasma are sampled to assess the comorbid metabolic syndrome. In <jats:italic>dp\/+<\/jats:italic> mice, histopathologic analyses reveal hypertrophic adipocytes and hepatic steatosis; serological examinations show hyperlipemia and hyperinsulinemia. Further, by comparing lipidomic and transcriptomic profiling of epididymal WAT between <jats:italic>dp\/+<\/jats:italic> and WT mice, the study finds the triglyceride (TG) accumulation in <jats:italic>dp\/+<\/jats:italic> mice in association with the dysfunction of lipid droplets. Validation of TG\u2010metabolism\u2010associated genes in WAT and in primary cultured adipocytes show enhanced TG synthesis and declined TG hydrolysis in the <jats:italic>dp\/+<\/jats:italic> model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study elucidates that the imbalanced TG synthesis\/hydrolysis in adipocytic lipid droplets may contribute to the hypertrophic obesity and metabolic disorders in mice with 16p11.2 microduplication.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202100241","type":"journal-article","created":{"date-parts":[[2022,1,24]],"date-time":"2022-01-24T16:04:10Z","timestamp":1643040250000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Microduplication of 16p11.2 <i>locus<\/i> Potentiates Hypertrophic Obesity in Association with Imbalanced Triglyceride Metabolism in White Adipose 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