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ALI is induced by giving 20% ethanol ad libitum combined with four \u201cbinges\u201d. Lf can remarkably decrease EtOH\u2010induced mortality. Lf promotes aldehyde dehydrogenase\u20102 (ALDH2) expression and suppressing cytochrome P450 2E1 (CYP2E1) overexpression, resulting in the reduced hepatic superoxide and inflammation levels, which ultimately leads to the hepatic injury alleviation. However, HLf increases acetyl\u2010CoA carboxylase and fatty acid synthase protein levels, which suggests that excessive intake may weaken the beneficial effects of Lf. Moreover, LLf increases the relative abundances of <jats:italic>Akkermansia<\/jats:italic> and <jats:italic>Lactobacillus<\/jats:italic>. Additionally, the study shows that Lf likely exerts action in its digestive product forms rather than intact Lf molecular in normal condition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>LLf can ameliorate ALI, which is associated with the regulation of hepatic alcohol metabolism and the modulation of gut microbiota. However, excessive Lf intake may result in a diminished benefit.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202100253","type":"journal-article","created":{"date-parts":[[2021,7,31]],"date-time":"2021-07-31T06:14:39Z","timestamp":1627712079000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Daily Dose of Bovine Lactoferrin Prevents Ethanol\u2010Induced Liver Injury and Death in Male Mice by Regulating Hepatic Alcohol Metabolism and Modulating Gut Microbiota"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8681-2752","authenticated-orcid":false,"given":"Deming","family":"Li","sequence":"first","affiliation":[{"name":"School of Public Health Medical College of Soochow University  199 Renai Road Suzhou Jiangsu 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"He","sequence":"additional","affiliation":[{"name":"School of Public Health Medical College of Soochow University  199 Renai Road Suzhou Jiangsu 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanhuan","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Public Health Medical College of Soochow University  199 Renai Road Suzhou Jiangsu 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yafang","family":"Du","sequence":"additional","affiliation":[{"name":"School of Public Health Medical College of Soochow University  199 Renai Road Suzhou Jiangsu 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kangqing","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Optics and Electronics Chinese Academy of Science  Chengdu Sichuan 610209 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition The First Affiliated Hospital of Soochow University  Suzhou 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Tong","sequence":"additional","affiliation":[{"name":"Laboratory Center Medical College of Soochow University  Suzhou 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaxin","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Public Health Medical College of Soochow University  199 Renai Road Suzhou Jiangsu 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaying","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Radiation Medicine and Protection School of Radiation Medicine and Protection Soochow University  Suzhou 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5231-2562","authenticated-orcid":false,"given":"Liqiang","family":"Qin","sequence":"additional","affiliation":[{"name":"School of Public Health Medical College of Soochow University  199 Renai Road Suzhou Jiangsu 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,10]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2017.469"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2019.2276"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2019.03.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.03.005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2015.35"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2013"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.10.037"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-018-0099-1"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2016.11.008"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2014.10.042"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2017.1381583"},{"key":"e_1_2_9_12_1","first-page":"10","author":"Min Q. 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