{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T12:17:59Z","timestamp":1762604279693,"version":"3.41.2"},"reference-count":33,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T00:00:00Z","timestamp":1629331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Food allergy to sunflower seed (SFS) protein is not frequent and only non\u2010specific lipid transfert protein (nsLTP) Hel a 3 is officially recognized as a food allergen. Out of the eleven seed storage 2S\u2010albumins (SESA) detected in SFS, only SFA\u20108 allergenicity has been investigated so far. The study aimed then to evaluate SFS protein allergenicity and particularly, to compare the sensitization potency of SESA in a mouse model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>The most abundant SESA and nsLTP were isolated from SFS through a combination of chromatographic methods. Purified proteins were then used to measure specific IgG1 and IgE responses in BALB\/c mice orally sensitized to different SFS protein isolates. The study, thus, confirmed the allergenicity of SFA\u20108 and Hel a 3 but mice were also highly sensitized to other SESA such as SESA2\u20101 or SESA20\u20102. Furthermore, competitive inhibition of IgE\u2010binding revealed that SFA\u20108 IgE\u2010reactivity was due to cross\u2010reactivity with other SESA. 11S\u2010globulins were weakly immunogenic and were rapidly degraded in an in vitro model of gastroduodenal digestion. In contrast, Hel a 3, SESA2\u20101 and SFA\u20108 were more resistant to proteolysis and gastroduodenal digestion did not affect their IgE\u2010reactivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>SESA2\u20101 or SESA20\u20102 were more potent allergens than SFA\u20108 in this mouse model. Allergenicity of SESA must be now confirmed in SFS\u2010allergic patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202100369","type":"journal-article","created":{"date-parts":[[2021,7,31]],"date-time":"2021-07-31T08:41:28Z","timestamp":1627720888000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Sensitization Potency of Sunflower Seed Protein in a Mouse Model: Identification of 2S\u2010Albumins More Allergenic Than SFA\u20108"],"prefix":"10.1002","volume":"65","author":[{"given":"Jihana","family":"Achour","sequence":"first","affiliation":[{"name":"CEA INRAE D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9 (DMTS)\/Service de Pharmacologie et d'Immunoanalyse Universit\u00e9 Paris\u2010Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marine","family":"Guinot","sequence":"additional","affiliation":[{"name":"CEA INRAE D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9 (DMTS)\/Service de Pharmacologie et d'Immunoanalyse Universit\u00e9 Paris\u2010Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Blanche","family":"Guillon","sequence":"additional","affiliation":[{"name":"CEA INRAE D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9 (DMTS)\/Service de Pharmacologie et d'Immunoanalyse Universit\u00e9 Paris\u2010Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romain","family":"Kapel","sequence":"additional","affiliation":[{"name":"Laboratoire R\u00e9actions et G\u00e9nie des Proc\u00e9d\u00e9s CNRS LRGP Universit\u00e9 de Lorraine  Nancy France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Galet","sequence":"additional","affiliation":[{"name":"Avril SCA  11\/13, rue de Monceau Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine","family":"Adel\u2010Patient","sequence":"additional","affiliation":[{"name":"CEA INRAE D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9 (DMTS)\/Service de Pharmacologie et d'Immunoanalyse Universit\u00e9 Paris\u2010Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4828-7031","authenticated-orcid":false,"given":"St\u00e9phane","family":"Hazebrouck","sequence":"additional","affiliation":[{"name":"CEA INRAE D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9 (DMTS)\/Service de Pharmacologie et d'Immunoanalyse Universit\u00e9 Paris\u2010Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3971-1021","authenticated-orcid":false,"given":"Herv\u00e9","family":"Bernard","sequence":"additional","affiliation":[{"name":"CEA INRAE D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9 (DMTS)\/Service de Pharmacologie et d'Immunoanalyse Universit\u00e9 Paris\u2010Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,19]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.2134\/agronmonogr35"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/nxz279"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01677856"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(90)87080-E"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-015-2653-3"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2016.05.004"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf100554d"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0486388"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf950683f"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2010.02346.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12868"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.12.976"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02155.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13223-014-0065-6"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(79)90107-6"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2010.02337.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.1995.tb00256.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2019.02.044"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/0394632016651648"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2000.00498.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.3.1561"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.11.438"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13601-016-0110-2"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.03.003"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028917"},{"key":"e_1_2_9_26_1","volume":"7","author":"Rodriguez M. J.","year":"2017","journal-title":"Sci Rep\u2010Uk"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2009.03312.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.12.034"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks400"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.126423"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12411"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201500923"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2009.08.010"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.202100369","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mnfr.202100369","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.202100369","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T19:43:06Z","timestamp":1693338186000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.202100369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,19]]},"references-count":33,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1002\/mnfr.202100369"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.202100369","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"type":"print","value":"1613-4125"},{"type":"electronic","value":"1613-4133"}],"subject":[],"published":{"date-parts":[[2021,8,19]]},"assertion":[{"value":"2021-04-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2100369"}}