{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T17:45:59Z","timestamp":1769017559588,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T00:00:00Z","timestamp":1624406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871381"],"award-info":[{"award-number":["31871381"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["91957114"],"award-info":[{"award-number":["91957114"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>DNA methylation contributes to obesity, but the role of the DNA demethylase ten\u2010eleven translocation protein 1 (Tet1) in obesity remains unclear. Vitamin C is a cofactor for the Tet family of proteins, but whether vitamin C can be used to treat obesity via Tet1 awaits clarification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p><jats:italic>Tet1<jats:sup>+\/+<\/jats:sup><\/jats:italic> and <jats:italic>Tet1<jats:sup>+\/\u2212<\/jats:sup><\/jats:italic> mice are fed a high fat diet (HFD). Higher weight gain and more severe hepatic steatosis, accompanied by reduced 5\u2010hydromethylcytosine (5hmC) levels, are found in the white adipose tissue and liver of <jats:italic>Tet1<jats:sup>+\/\u2212<\/jats:sup><\/jats:italic> mice. Accumulated lipids are observed in palmitic acid or oleic acid treated primary hepatocytes derived from <jats:italic>Tet1<jats:sup>+\/\u2212<\/jats:sup><\/jats:italic> mice, which are rescued by Tet1 overexpression or vitamin C treatment. Bisulfite sequencing reveals higher DNA methylation levels on lipolysis related genes in the liver of <jats:italic>Tet1<jats:sup>+\/\u2212<\/jats:sup><\/jats:italic> mice. Notably, oral intake of vitamin C normalizes DNA methylation levels, promotes lipolysis, and decreases obesity in HFD\u2010fed <jats:italic>Tet1<jats:sup>+\/\u2212<\/jats:sup><\/jats:italic> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results reveal a novel function of Tet1 in obesity and provide a new mechanism for the beneficial role of vitamin C in metabolic diseases through enhanced Tet1 activity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202100417","type":"journal-article","created":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T12:47:03Z","timestamp":1623761223000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Vitamin C Inhibits the Metabolic Changes Induced by Tet1 Insufficiency Under High Fat Diet Stress"],"prefix":"10.1002","volume":"65","author":[{"given":"Yangmian","family":"Yuan","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Cell Homeostasis College of Life Sciences Wuhan University  Wuhan 430072 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Wuhan Hospital of Traditional Chinese and Western Medicine Tongji Medical College Huazhong University of Science and Technology  Wuhan 430030 China"},{"name":"Tongji School of Pharmacy Tongji Medical College Huazhong University of Science and Technology  Wuhan 430030 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingrui","family":"Chen","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Cell Homeostasis College of Life Sciences Wuhan University  Wuhan 430072 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuyan","family":"Sun","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Cell Homeostasis College of Life Sciences Wuhan University  Wuhan 430072 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingrui","family":"Xiong","sequence":"additional","affiliation":[{"name":"Tongji School of Pharmacy Tongji Medical College Huazhong University of Science and Technology  Wuhan 430030 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Fan","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Cell Homeostasis College of Life Sciences Wuhan University  Wuhan 430072 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert B.","family":"Petersen","sequence":"additional","affiliation":[{"name":"Foundational Sciences Central Michigan University College of Medicine  Mt. 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