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The expression of nuclear factor erythroid 2\u2010related factor 2 (Nrf2), superoxide dismutase 1 (SOD1), and NAD(P)H quinone dehydrogenase 1 (NQO1) decreases significantly in the DIO and DIO\u2010R groups; m6A levels in testis tissue in the DIO and DIO\u2010R groups increase; the enrichment of m6A\u2010modified Nrf2 mRNA in testis in the DIO group and DIO\u2010R group increases significantly. Also the m6A regulatory proteins increase significantly in the DIO group and DIO\u2010R group. In vitro, compared to palmitic acid treated cells, the reactive oxygen species (ROS) level significantly decreases in STM2457, S\u2010Adenosylhomocysteine treated cells and YTHDC2, YTHDF2 gene silence cells; however, Nrf2 expression increases in all treated cells. In addition, m6A expression decreases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Oxidative stress mediates by methylation of m6A may contribute to high fat diet\u2010induced male reproductive dysfunction.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202101052","type":"journal-article","created":{"date-parts":[[2023,2,4]],"date-time":"2023-02-04T06:03:40Z","timestamp":1675490620000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Oxidative Stress Mediated by N6\u2010Methyladenosine Methylation Contributes to High\u2010Fat Diet Induced Male Reproductive Dysfunction"],"prefix":"10.1002","volume":"67","author":[{"given":"Jingyi","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Pharmacology Shenyang Pharmaceutical University  No.103, Wenhua Rd, Shenhe Dist Shenyang 110016 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mujiao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Shenyang Pharmaceutical University  No.103, Wenhua Rd, Shenhe Dist Shenyang 110016 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Shenyang Pharmaceutical University  No.103, Wenhua Rd, Shenhe Dist Shenyang 110016 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Maternal and Child Health School of Public Health China Medical University  No.77 Puhe Road, Shenbei District Shenyang 110122 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9755-5587","authenticated-orcid":false,"given":"Jian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Shenyang Pharmaceutical University  No.103, Wenhua Rd, Shenhe Dist Shenyang 110016 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,16]]},"reference":[{"key":"e_1_2_8_1_1","unstructured":"https:\/\/www.who.int\/news\u2010room\/fact\u2010sheets\/detail\/obesity\u2010and\u2010overweight"},{"key":"e_1_2_8_2_1","unstructured":"https:\/\/www.who.int\/health\u2010topics\/obesity#tab=tab_1"},{"key":"e_1_2_8_3_1","volume":"2021","author":"Leisegang K.","year":"2021","journal-title":"Andrologia"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmu042"},{"key":"e_1_2_8_5_1","volume-title":"Obesity: Preventing and Managing the Global Epidemic","author":"WHO Consultation on Obesity","year":"2000"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmx006"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.001180"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph16193759"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.2014.32.1.1"},{"key":"e_1_2_8_10_1","volume":"201","author":"Zhao J.","year":"2014","journal-title":"Oxid. 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