{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T14:42:23Z","timestamp":1787582543469,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T00:00:00Z","timestamp":1647734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31601472"],"award-info":[{"award-number":["31601472"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:sec>\n                    <jats:title>Scope<\/jats:title>\n                    <jats:p>Iron deficiency anemia (IDA) in children is one of the most common nutrition\u2010related health conditions worldwide. Prebiotic oligosaccharides, fructo\u2010oligosaccharide (FOS) and galacto\u2010oligosaccharides (GOS), have shown to affect iron absorption in anemic subjects, but the results in previous studies are inconsistent, thus the underlying mechanism and the effective dose of GOS in mitigating anemia remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>The present study aims to investigate the mechanism of how GOS\/FOS affect iron absorption in an iron\u2010deficient growing rat model from the perspectives of protein expression and gut microbiota, and determine the optimum dose of GOS. Iron\u2010deficient models are established by providing young rats diet without iron addition for 14 days. Later, iron\u2010deficient rats are provided with standard rat chows supplemented with 0%, 3%, 5%, 10% GOS, and 10% FOS for 21 days. The results show that \u22655% GOS supplementation in diet improves iron status and significantly impacts iron\u2010binding\/transport protein expression. Furthermore, a dose\u2010dependent modulating effect of GOS on gut microbiota is determined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>For the first time, the present study provides evidence that GOS supplementation induces a dose\u2010response effect on iron absorption and gut microbiota in the established model, suggesting a positive role of GOS in ameliorating IDA in children.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mnfr.202101064","type":"journal-article","created":{"date-parts":[[2022,2,20]],"date-time":"2022-02-20T04:41:09Z","timestamp":1645332069000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Prebiotic Oligosaccharides Enhance Iron Absorption Via Modulation of Protein Expression and Gut Microbiota in a Dose\u2010Response Manner in Iron\u2010Deficient Growing Rats"],"prefix":"10.1002","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7243-4444","authenticated-orcid":false,"given":"Jie\u2010Hua","family":"Chen","sequence":"first","affiliation":[{"name":"Institute for Innovative Development of Food Industry Shenzhen University  Shenzhen 518060 China"},{"name":"Shenzhen Key Laboratory of Marine Microbiome Engineering Institute for Advanced Study Shenzhen University  Shenzhen 518060 China"},{"name":"Department of Nutrition and Food Hygiene School of Public Health Southern Medical University  Guangzhou 510515 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene School of Public Health Southern Medical University  Guangzhou 510515 China"},{"name":"Zhuhai Maternity and Child Health Hospital  Zhuhai 519001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialiang","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Inspection and Quarantine School of Public Health Southern Medical University  Guangzhou 510515 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise Weiwei","family":"Lu","sequence":"additional","affiliation":[{"name":"Human Nutrition Unit School of Biological Sciences University of Auckland  Auckland 1010 New Zealand"},{"name":"High Value Nutrition National Science Challenge  Auckland 1142 New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute for Innovative Development of Food Industry Shenzhen University  Shenzhen 518060 China"},{"name":"Shenzhen Key Laboratory of Marine Microbiome Engineering Institute for Advanced Study Shenzhen University  Shenzhen 518060 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute for Innovative Development of Food Industry Shenzhen University  Shenzhen 518060 China"},{"name":"Shenzhen Key Laboratory of Marine Microbiome Engineering Institute for Advanced Study Shenzhen University  Shenzhen 518060 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute for Innovative Development of Food Industry Shenzhen University  Shenzhen 518060 China"},{"name":"Shenzhen Key Laboratory of Marine Microbiome Engineering Institute for Advanced Study Shenzhen University  Shenzhen 518060 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene School of Public Health Southern Medical University  Guangzhou 510515 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,20]]},"reference":[{"key":"e_1_2_9_1_1","volume-title":"Advanced nutrition and human metabolism","author":"Gropper S. 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