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This study aims to evaluate the effects of regular consumption of WP on constipation\u2010induced complications and gut microbiota in humans and mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>A randomized trial of 49 functional constipation participants is conducted. The weekly amount of spontaneous bowel movements (SBM) increases by 2.09 per week after WP treatment, and by 0.40 per week among the placebo group (PL). Concomitantly, the secondary outcomes show significant improvements in the quality of life\u2010related to constipation (PAC\u2010QOL), constipation severity, and satisfaction with the intervention. In the animal study, WP effectively alleviates constipation symptoms and affects the secretion of intestinal mobility\u2010related neurotransmitters and gastrointestinal hormones in loperamide\u2010induced constipation mice. Additionally, WP regulates the gene and protein expression levels of water\u2010electrolyte metabolism and intestinal mobility. Furthermore, WP treatment decreases the abundance of several gut microbiota positively correlated to constipation (<jats:italic>Turicibacter, Bacteroides_f_Bacteroidaceae, and Streptococcus<\/jats:italic>) in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>WP ameliorates constipation in humans and mice, which could be partly explained by improving water\u2010electrolyte metabolism, boosting intestinal motility, and reshaping gut microbiota.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202200313","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T10:14:03Z","timestamp":1659521643000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Consumption of Wheat Peptides Improves Functional Constipation: A Translational Study in Humans and Mice"],"prefix":"10.1002","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6283-2257","authenticated-orcid":false,"given":"Qianqian","family":"Wang","sequence":"first","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3599-2682","authenticated-orcid":false,"given":"Junhui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiying","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Biological &amp; Chemical Engineering Zhejiang University of Science &amp; Technology  Hangzhou 310023 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Pan","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengjia","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihang","family":"Gong","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Du","sequence":"additional","affiliation":[{"name":"Hangzhou Kangyuan Food Science &amp; Technology Co., Ltd.  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