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Of note, upregulation of miR\u201029a ameliorates high\u2010fat diet (HFD)\u2010induced liver dysfunctions in mice. However, the miR\u201029a effect on gut microbiome composition and HFD\u2010induced gut microbiota changes during metabolic disturbances remains unclear. The study provides compelling evidence for the protective role of miR\u201029a in gut barrier dysfunction and steatohepatitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>miR\u201029a overexpressed mice (miR\u201029aTg) are bred to characterize intestinal, serum biochemical, and fecal microbiota profiling features compared to wild\u2010type mice (WT). Mice are fed an HFD for 8 months to induce steatohepatitis, and intestinal dysfunction is determined via histopathological analysis. miR\u201029aTg has better lipid metabolism capability that decreases total cholesterol and triglyceride levels in serum than WT of the same age. The study further demonstrates that miR\u201029aTg contributes to intestinal integrity by maintaining periodic acid Schiff positive cell numbers and diversity of fecal microorganisms. HFD\u2010induced bacterial community disturbance and steatohepatitis result in more severe WT than miR\u201029aTg. Gut microorganism profiling reveals <jats:italic>Lactobacillus<\/jats:italic>, <jats:italic>Ruminiclostridium_9<\/jats:italic>, and <jats:italic>Lachnoclostridium<\/jats:italic> enrichment in miR\u201029aTg and significantly decreases interleukin\u20106 expression in the liver and intestinal tract.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides new evidence that sheds light on the host genetic background of miR\u201029a, which protects against steatohepatitis and other intestinal disorders.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202200348","type":"journal-article","created":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T08:31:00Z","timestamp":1682757060000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["MicroRNA\u201029a Compromises Hepatic Adiposis and Gut Dysbiosis in High Fat Diet\u2010Fed Mice via Downregulating Inflammation"],"prefix":"10.1002","volume":"67","author":[{"given":"Ya\u2010Ling","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Kaohsiung Chang Gung Memorial Hospital  Kaohsiung 833 Taiwan"},{"name":"Chang Gung University College of Medicine  Taoyuan 333 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying\u2010Hsien","family":"Huang","sequence":"additional","affiliation":[{"name":"Chang Gung University College of Medicine  Taoyuan 333 Taiwan"},{"name":"Department of Pediatrics Kaohsiung Chang Gung Memorial Hospital Chang  Kaohsiung 833 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng\u2010Sheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Mitochondrial Research and Medicine Kaohsiung Chang Gung Memorial Hospital  Kaohsiung 833 Taiwan"},{"name":"Core Laboratory for Phenomics &amp; Diagnostics Department of Medical Research Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine  Kaohsiung 833 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010Chao","family":"Tsai","sequence":"additional","affiliation":[{"name":"Chang Gung University College of Medicine  Taoyuan 333 Taiwan"},{"name":"Division of Hepato\u2010Gastroenterology Department of Internal Medicine Kaohsiung Chang Gung Memorial Hospital  Kaohsiung 833 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chien\u2010Hung","family":"Chen","sequence":"additional","affiliation":[{"name":"Chang Gung University College of Medicine  Taoyuan 333 Taiwan"},{"name":"Division of Hepato\u2010Gastroenterology Department of Internal Medicine Kaohsiung Chang Gung Memorial Hospital  Kaohsiung 833 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3462-3620","authenticated-orcid":false,"given":"Wei\u2010Shiung","family":"Lian","sequence":"additional","affiliation":[{"name":"Center for Mitochondrial Research and Medicine Kaohsiung Chang Gung Memorial Hospital  Kaohsiung 833 Taiwan"},{"name":"Core Laboratory for Phenomics &amp; Diagnostics Department of Medical Research Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine  Kaohsiung 833 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,15]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.774335"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0433-9"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2014.01.020"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2021.04.011"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0569-4"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/advances\/nmy078"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecco-jcc\/jjab223"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2019.12.005"},{"key":"e_1_2_10_9_1","first-page":"8","author":"Contijoch E. 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