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Piglets in the NAC+PEDV group are orally administrated with NAC (100\u00a0mg\u00a0(kg\u00b7BW)<jats:sup>\u22121<\/jats:sup>\u00a0day<jats:sup>\u22121<\/jats:sup>) for 4 consecutive days after 2 days of PEDV infection. The results show that NAC administration decreases the diarrhea rate and improves intestinal morphology. The concentration of diamine oxidase and intestinal fatty\u2010acid binding protein, as well as IL\u20101\u03b2, IL\u20108, and TNF\u2010\u03b1 in the plasma, is decreased by NAC. Intriguingly, NAC administration significantly increases the viral load in the jejunum and ileum and down\u2010regulates the expression of interferon\u2010related genes. Microarray and proteomic analyses show that the differentially expressed genes\/proteins between NAC+PEDV and PEDV groups are highly enriched in substance transport. Furthermore, aquaporin 8\/10 expression is significantly increased by NAC upon PEDV infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>NAC administration alleviates PEDV\u2010induced intestinal injury by inhibiting inflammatory responses and improving substance transport, but promotes viral replication by inhibiting interferon signaling. These results suggest NAC exhibits multifaceted effects upon PEDV infection, and thus caution is required when using NAC as a dietary supplement to prevent viral infection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202200369","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T09:44:06Z","timestamp":1667382246000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Multifaceted Effects and Mechanisms of N\u2010Acetylcysteine on Intestinal Injury in a Porcine Epidemic Diarrhea Virus\u2010Infected Porcine Model"],"prefix":"10.1002","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0747-1422","authenticated-orcid":false,"given":"Qian","family":"Zhang","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science Wuhan Polytechnic University  Wuhan 430023 P. R. 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