{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:49:41Z","timestamp":1790113781719,"version":"4.0.1"},"reference-count":67,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:sec>\n                    <jats:title>Scope<\/jats:title>\n                    <jats:p>Obesity is a global threat for male infertility, which can cause spermatogenic dysfunction. However, there are no available drugs for the treatment of obesity\u2010induced spermatogenesis dysfunction. This study characterizes the protective effects of icariin (ICA) on spermatogenesis dysfunction in obese mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>\n                      Obese mice are induced by a high\u2010fat diet to determine whether ICA has a protective effect. ICA treatment reduces body weight and the proportion of abnormal sperm, brings about a recovery of sperm count, and the number of spermatogenic cells. ICA treatment improves histopathological changes of the testes and inhibits testicular apoptosis, as evidenced by reduced the expression of Bax and increased the expression of Bcl\u20102, PCNA, WT1, GATA\u20104, vimentin, HK2, PKM2, and LDHA in the testes. In vitro, TM4 cells are treated with 0.4\u00a0m\n                      <jats:sc>m<\/jats:sc>\n                      palmitic acid (PA) to induce Sertoli cell injury, and are then utilized for ICA treatment. ICA improves PA\u2010induced decreased TM4 cells viability, reduces the levels of lactate, and increases the levels of pyruvate and the expression of HK2, PKM2, and LDHA and restores the glycolytic process in vitro.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>ICA ameliorates spermatogenic dysfunction in obese mice by regulating glycolytic activity, providing effective strategies for obesity treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mnfr.202200524","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T04:50:13Z","timestamp":1681447813000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Icariin Ameliorates Spermatogenesis Disorder in Obese Mice Induced by High\u2010Fat Diet through Regulating the Glycolytic Pathway"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5300-3462","authenticated-orcid":false,"given":"Min","family":"Luo","sequence":"first","affiliation":[{"name":"Reproductive Hospital of Guangxi Zhuang Autonomous Region  Nanning 530021 China"},{"name":"Clinical Anatomy and Reproductive Medicine Application Institute Department of Histology and Embryology Hengyang Medical School University of South China  Hengyang 421001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuhong","family":"Zhuge","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Guilin Medical University  Guilin Guangxi 541001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Ji","sequence":"additional","affiliation":[{"name":"Reproductive Hospital of Guangxi Zhuang Autonomous Region  Nanning 530021 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Clinical Anatomy and Reproductive Medicine Application Institute Department of Histology and Embryology Hengyang Medical School University of South China  Hengyang 421001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Mo","sequence":"additional","affiliation":[{"name":"Reproductive Hospital of Guangxi Zhuang Autonomous Region  Nanning 530021 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongpeng","family":"Tan","sequence":"additional","affiliation":[{"name":"Clinical Anatomy and Reproductive Medicine Application Institute Department of Histology and Embryology Hengyang Medical School University of South China  Hengyang 421001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leguang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Clinical Anatomy and Reproductive Medicine Application Institute Department of Histology and Embryology Hengyang Medical School University of South China  Hengyang 421001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaocan","family":"Lei","sequence":"additional","affiliation":[{"name":"Clinical Anatomy and Reproductive Medicine Application Institute Department of Histology and Embryology Hengyang Medical School University of South China  Hengyang 421001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Huang","sequence":"additional","affiliation":[{"name":"Reproductive Hospital of Guangxi Zhuang Autonomous Region  Nanning 530021 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6]]},"reference":[{"key":"e_1_2_9_1_1","first-page":"31","author":"Djalalinia S.","year":"2015","journal-title":"Pak. 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