{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T14:17:12Z","timestamp":1779891432377,"version":"3.53.1"},"reference-count":66,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T00:00:00Z","timestamp":1695945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32260851"],"award-info":[{"award-number":["32260851"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32202707"],"award-info":[{"award-number":["32202707"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Puerarin has possessed a wide range of pharmacological activities. However, little is known about the protective effects of puerarin on the oxidized oil\u2010induced injury. Here, the antioxidant and anti\u2010inflammatory effects of puerarin are described using a chicken model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>A total of 360 broilers are arranged in four treatments. Diets include two types of soybean oil (fresh or oxidized) and two levels of puerarin (0 or 750 mg\u00a0kg<jats:sup>\u22121<\/jats:sup>). Results show that puerarin alleviates oxidized soybean oil\u2010induced hepatic and thymic oxidative injury. This effect is observed by increasing the SOD activity and the expressions of Nrf2 signaling pathway\u2010related genes and reducing the MDA content in the liver and thymus. Moreover, puerarin supplementation decreases the concentrations and mRNA levels of pro\u2010inflammatory factors in the liver and thymus. The potential mechanism responsible for this is the decrease in the mRNA or protein levels of HMGB1, TLR4, MyD88, and p65 in the liver or thymus. Western blotting results indicate that puerarin also decreases the phosphorylation of JNK1\/2, ERK1\/2, and p38 in the liver and thymus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrates puerarin may be a potential nutrient supplement in the treatment of oxidized oil\u2010induced damage, and the Nrf2\/Keap1 and HMGB1\/TLR4\/MAPK signaling pathways might be its important target.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202200663","type":"journal-article","created":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T06:54:48Z","timestamp":1696056888000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Puerarin Alleviates Oxidized Oil\u2010Induced Oxidative Injury and Inflammation via Inhibition of the Nrf2\/Keap1 and HMGB1\/TLR4\/MAPK Signaling Pathways: An Investigation in a Chicken Model"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2581-4276","authenticated-orcid":false,"given":"Hua","family":"Zhou","sequence":"first","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology Jiangxi Agricultural University  Nanchang 330045 P. R. China"},{"name":"Jiangxi Province Key Innovation Center of Integration in Production and Education for High\u2010quality and Safe Livestock and Poultry  Nanchang 330045 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Shi","sequence":"additional","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology Jiangxi Agricultural University  Nanchang 330045 P. R. China"},{"name":"Jiangxi Province Key Innovation Center of Integration in Production and Education for High\u2010quality and Safe Livestock and Poultry  Nanchang 330045 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingmei","family":"Yu","sequence":"additional","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology Jiangxi Agricultural University  Nanchang 330045 P. R. China"},{"name":"Jiangxi Province Key Innovation Center of Integration in Production and Education for High\u2010quality and Safe Livestock and Poultry  Nanchang 330045 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology Jiangxi Agricultural University  Nanchang 330045 P. R. China"},{"name":"Jiangxi Province Key Innovation Center of Integration in Production and Education for High\u2010quality and Safe Livestock and Poultry  Nanchang 330045 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingxin","family":"OuYang","sequence":"additional","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology Jiangxi Agricultural University  Nanchang 330045 P. R. China"},{"name":"Jiangxi Province Key Innovation Center of Integration in Production and Education for High\u2010quality and Safe Livestock and Poultry  Nanchang 330045 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinhao","family":"Bian","sequence":"additional","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology Jiangxi Agricultural University  Nanchang 330045 P. R. China"},{"name":"Jiangxi Province Key Innovation Center of Integration in Production and Education for High\u2010quality and Safe Livestock and Poultry  Nanchang 330045 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichun","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology Jiangxi Agricultural University  Nanchang 330045 P. R. China"},{"name":"Jiangxi Province Key Innovation Center of Integration in Production and Education for High\u2010quality and Safe Livestock and Poultry  Nanchang 330045 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3589-9023","authenticated-orcid":false,"given":"Guanhong","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology Jiangxi Agricultural University  Nanchang 330045 P. R. China"},{"name":"Jiangxi Province Key Innovation Center of Integration in Production and Education for High\u2010quality and Safe Livestock and Poultry  Nanchang 330045 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,29]]},"reference":[{"key":"e_1_2_8_1_1","first-page":"173","volume":"24","author":"Anjum M.","year":"2004","journal-title":"Vet. J."},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2010-01180"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2011-01354"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(03)00151-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M026179"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf102918z"},{"key":"e_1_2_8_7_1","first-page":"1541","volume":"16","author":"Shafaeizadeh S.","year":"2011","journal-title":"J. Res. Med. Sci."},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf903622q"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.4311"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-011-0181-8"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-5266"},{"key":"e_1_2_8_12_1","first-page":"957","volume":"10","author":"Li L.","year":"2018","journal-title":"Am. J. Transl. Res."},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcph.96"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5083"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2015-1213"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2016.06.008"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5148"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2017.4922"},{"key":"e_1_2_8_19_1","first-page":"1610","volume":"40","author":"Zheng G. M.","year":"2012","journal-title":"Int. J. Oncol."},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.896058"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21347"},{"key":"e_1_2_8_22_1","first-page":"3203","volume":"8","author":"Liu X.","year":"2013","journal-title":"Neural Regen. Res."},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.14.0924"},{"key":"e_1_2_8_24_1","unstructured":"MOH; SAC. Determination of Peroxide Value in Food. In GB 5009.227\u20102016 Ministry of Health and Standardization Administration of China Beijing China2016."},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2001.281.5.E908"},{"key":"e_1_2_8_27_1","volume-title":"Dissertations","author":"Lindblom S. C.","year":"2017"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8140962"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2007.11.021"},{"key":"e_1_2_8_30_1","first-page":"1416","volume":"18","author":"Dubey S.","year":"2018","journal-title":"Plant. Arch."},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.106993"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8FO02091D"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1665-2681(19)32099-X"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2009.11.004"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(02)01946-X"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2019.110126"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2008.12.021"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2005.7.1664"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.8b00374"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.11.006"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-85880-5"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00540"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/17474124.2020.1708191"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2020.01.021"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2011.07.008"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.14.0924"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X17500641"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.5357"},{"key":"e_1_2_8_49_1","first-page":"1","volume":"2017","author":"Yang R.","year":"2017","journal-title":"Mediators Inflamm."},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1080\/08830185.2016.1261318"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2017.10.006"},{"key":"e_1_2_8_52_1","first-page":"5125","volume":"17","author":"Shi Y.","year":"2018","journal-title":"Mol. Med. Rep."},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.02.037"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e16-02-0126"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01889"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12012-020-09571-9"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0171612"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.5357"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.03.018"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.04.176"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b07611"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-015-0242-x"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008207.2020.1778680"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.686992"},{"key":"e_1_2_8_65_1","first-page":"1","volume":"20","author":"Hu Y.","year":"2020","journal-title":"Exp. Ther. Med."},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2012.752"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.202200663","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,21]],"date-time":"2024-01-21T12:34:21Z","timestamp":1705840461000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.202200663"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,29]]},"references-count":66,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1002\/mnfr.202200663"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.202200663","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,29]]},"assertion":[{"value":"2022-09-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2200663"}}