{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T12:34:12Z","timestamp":1766579652260,"version":"3.41.2"},"reference-count":30,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T00:00:00Z","timestamp":1687824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Perinatal high\u2010fat diets (PHF) can influence fetal\/neonate development, resulting in cardiovascular pathogenesis, but precise mechanisms remain unclear. This study tests aldosterone receptor\u2010mediated Ca<jats:sup>2+<\/jats:sup> influx and the underlying mechanisms influenced by PHF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Maternal S.D. rats receive PHF during pregnancy and lactation periods. Their male offspring\u00a0are fed normal diets after weaning for four months. Mesenteric arteries (MA)\u00a0are for electrophysiological testing, Ca<jats:sup>2+<\/jats:sup> imaging, target gene expression, and promotor methylation. PHF increases aldosterone receptor gene Nr3c2\u2010mediated Ca<jats:sup>2+<\/jats:sup> currents in the smooth muscle cells (SMCs) of the MA via L\u2010type Ca<jats:sup>2+<\/jats:sup> channels (LTCC) in the offspring. The increased expression of aldosterone\u2010receptors and LTCC\u00a0are responsible for an activated Nr3c2\u2010LTCC pathway in the vasculature, eventually predisposes an increase of Ca<jats:sup>2+<\/jats:sup> influx in the myocytes of resistance arteries. The inhibitor of aldosterone\u2010receptors suppresses the increased Ca<jats:sup>2+<\/jats:sup> currents in the SMCs. Nr3c2 and LTCC\u00a0are upregulated through the transcriptional mechanism in methylation, which\u00a0can be reversed in the functional changes by methylation inhibitor 5AZA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results firstly demonstrate that aldosterone\u2010receptor activation can stimulate Ca<jats:sup>2+<\/jats:sup> currents via LTCC in vascular myocytes, which can be altered by perinatal foods via epigenetic changes of DNA methylation in the promoters of Nr3c2 and LTCC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202200722","type":"journal-article","created":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T11:23:32Z","timestamp":1687865012000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Fat\u2010Diets in Perinatal Stages Altered Nr3c2\u2010Mediated Ca<sup>2+<\/sup> Currents in Mesenteric Arteries of Offspring Rats"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2874-8470","authenticated-orcid":false,"given":"Qiutong","family":"Zheng","sequence":"first","affiliation":[{"name":"Maternal and Child Health Care Hospital of Wuxi &amp; 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