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This study elucidates the mechanism underlying the suppressive effect of <jats:sc>d<\/jats:sc>\u2010allulose on hepatic fat accumulation in terms of miRNA regulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Male C57BL\/6 mice are divided into three experimental groups\u2013normal diet and distilled water (CC group), high\u2010fat diet (HFD) and distilled water (HC group), and HFD and 5% <jats:sc>d<\/jats:sc>\u2010allulose\u00a0solution (HA group)\u2013and fed the respective diets for 8 weeks. Weight gain is significantly lower in the HA group than that in the HC group, although the caloric intake is the same in both. Histological analysis of liver tissues reveals excessive lipid accumulation in the HC group; this is greatly attenuated in the HA group. Real\u2010time PCR and western blot analyses demonstrate that, compared to the HC group, the HA group exhibits decreased hepatic PPAR\u03b3 and CD36 expression. Hepatic miR\u2010130 expression levels are higher in the HA group than those in the CC and HC groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results indicate that miRNA changes associated with PPAR\u03b3 may underlie the suppression of hepatic lipid accumulation induced by <jats:sc>d<\/jats:sc>\u2010allulose intake.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202200748","type":"journal-article","created":{"date-parts":[[2022,12,3]],"date-time":"2022-12-03T15:14:23Z","timestamp":1670080463000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["<scp>d<\/scp>\u2010Allulose Supplementation Prevents Diet\u2010Induced Hepatic Lipid Accumulation via miR\u2010130\u2010Mediated Regulation in C57BL\/6 Mice"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8170-9027","authenticated-orcid":false,"given":"Mirai","family":"Yamazaki","sequence":"first","affiliation":[{"name":"Department of Medical Technology Kagawa Prefectural University of Health Sciences  Takamatsu 761\u20100123 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misaki","family":"Okito","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Kagawa Prefectural University of Health Sciences  Takamatsu 761\u20100123 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Harada","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery Kagawa University Faculty of Medicine  Miki 761\u20100793 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Miyake","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery Kagawa University Faculty of Medicine  Miki 761\u20100793 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Tamiya","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery Kagawa University Faculty of Medicine  Miki 761\u20100793 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takehiro","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Physiology 2 Kawasaki Medical School  Kurashiki 701\u20100192 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,16]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2012.08.043"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-012-0533-z"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.171"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13098-020-00570-y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002043"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2009.07.018"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.48.77"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2006.04.016"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.881037"},{"key":"e_1_2_9_10_1","first-page":"1","volume":"128","author":"Smith A.","year":"2021","journal-title":"Br. 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