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Microorganisms may be a potent solution to this problem, but the mechanism is still unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Based on models in vivo and in vitro, the potential mechanism involving <jats:italic>Bifidobacterium animalis<\/jats:italic> F1\u20107 (<jats:italic>B. animalis<\/jats:italic> F1\u20107), screened in the previous studies, is explored through non\u2010targeted metabonomics, electrophysiological experiment and molecular level docking. The results showed that <jats:italic>B. animalis<\/jats:italic> F1\u20107 effectively alleviates OIC and promotes the expression of chromogranin A (CGA) and 5\u2010hydroxytryptamine (5\u2010HT). The metabolite 13,14\u2010dihydro\u201015\u2010keto\u2010PGE2 related to <jats:italic>B. animalis<\/jats:italic> F1\u20107 is found, which has a potential improvement effect on OIC at 20\u00a0mg kg BW<jats:sup>\u22121<\/jats:sup> in vivo. At 30\u00a0ng mL<jats:sup>\u22121<\/jats:sup> it effectively stimulates secretion of CGA\/5\u2010HT (408.95\u00a0\u00b1 1.18\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup>) by PC\u201012 cells and changes the membrane potential potassium ion current without affecting the sodium ion current in vitro. It upregulates the target of free fatty acid receptor\u20104 protein(FFAR4\/<jats:italic>\u03b2<\/jats:italic>\u2010actin, 0.81\u00a0\u00b1 0.02).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results demonstrate that metabolite 13,14\u2010dihydro\u201015\u2010keto\u2010PGE2 participated in <jats:italic>B. animalis<\/jats:italic> F1\u20107 to alleviate OIC via the 5\u2010HT pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202200846","type":"journal-article","created":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T18:23:45Z","timestamp":1701887025000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Metabolites 13,14\u2010Dihydro\u201015\u2010keto\u2010PGE2 Participates in <i>Bifidobacterium animalis<\/i> F1\u20107 to Alleviate Opioid\u2010Induced Constipation by 5\u2010HT Pathway"],"prefix":"10.1002","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2639-0604","authenticated-orcid":false,"given":"Youyou","family":"Lu","sequence":"first","affiliation":[{"name":"College of Food Science and Technology Huazhong Agricultural University  Wuhan 430070 China"},{"name":"Key Laboratory of Environment Correlative Dietology Ministry of Education (Huazhong Agricultural University)  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Qingdao Central Hospital  Qingdao 266042 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeting","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Huazhong Agricultural University  Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyu","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao 266000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao 266000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8968-4263","authenticated-orcid":false,"given":"Huaxi","family":"Yi","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao 266000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pimin","family":"Gong","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao 266000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3459-2975","authenticated-orcid":false,"given":"Lanwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Ocean University of China  Qingdao 266000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,6]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmn.2022.04.004"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurom.2022.06.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soncn.2019.04.003"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40263-020-00743-3"},{"key":"e_1_2_9_5_1","volume":"8","author":"Li Y.","year":"2021","journal-title":"Cochrane Database Syst. 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