{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T19:08:18Z","timestamp":1785784098703,"version":"3.56.0"},"reference-count":30,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T00:00:00Z","timestamp":1694649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pericles.pericles-gcp.literatumonline.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Malaria remains one of the most important infectious diseases in the world. Allyl isothiocyanate (AITC) is a main ingredient of traditional spice <jats:italic>Wasabia japonica<\/jats:italic>, which is reported to have anti\u2010bacterial and antiparasitic activities. However, there is no information on effects of AITC against malaria. The present study investigates the anti\u2010malarial activity of dietary AITC in vivo and that of AITC metabolites in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>The ad libitum administration of 35, 175, or 350 \u00b5M AITC\u2010containing drinking water to ICR mice significantly inhibit the parasitemia induced after infection with <jats:italic>Plasmodium berghei<\/jats:italic>. On the other hand, after single oral administration of AITC (20\u00a0mg\u00a0kg<jats:sup>\u22121<\/jats:sup> body weight), <jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:italic>S<\/jats:italic>\u2010(<jats:italic>N<\/jats:italic>\u2010allylthiocarbamoyl)\u2010<jats:sc>l<\/jats:sc>\u2010cysteine (NAC\u2010AITC) as one of the AITC metabolites displays a serum <jats:italic>C<\/jats:italic><jats:sub>max<\/jats:sub> of 11.4\u00a0\u00b5M at a <jats:italic>T<\/jats:italic><jats:sub>max<\/jats:sub> of 0.5\u00a0h, but AITC is not detected at any time point. Moreover, NAC\u2010AITC shows anti\u2010malarial activity against <jats:italic>Plasmodium falciparum<\/jats:italic> in vitro, and its 50% inhibitory concentration (IC<jats:sub>50<\/jats:sub>) against parasitemia is 12.6\u00a0\u00b5M.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results indicate that orally administered AITC is metabolized to NAC\u2010AITC and exerts anti\u2010malarial activity against malaria parasites in blood, suggesting that the consumption of AITC\u2010containing food stuffs such as cruciferous plants may prevent malaria.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202300185","type":"journal-article","created":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T17:27:21Z","timestamp":1694712441000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Anti\u2010Malarial Activity of Allyl Isothiocyanate and <i>N<\/i>\u2010acetyl\u2010<i>S<\/i>\u2010(<i>N<\/i>\u2010allylthiocarbamoyl)\u2010<scp>l<\/scp>\u2010Cysteine"],"prefix":"10.1002","volume":"67","author":[{"given":"Takashi","family":"Hashimoto","sequence":"first","affiliation":[{"name":"Department of Agrobioscience Graduate School of Agricultural Science Kobe University  1\u20101 Rokkodai, Nada Kobe Hyogo 657\u20108501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoji","family":"Yoshioka","sequence":"additional","affiliation":[{"name":"Department of Agrobioscience Graduate School of Agricultural Science Kobe University  1\u20101 Rokkodai, Nada Kobe Hyogo 657\u20108501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiroh","family":"Iwanaga","sequence":"additional","affiliation":[{"name":"Department of Molecular Protozoology Research Institute for Microbial Diseases Osaka University  3\u20101 Yamadaoka Suita Osaka 565\u20100871 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuki","family":"Kanazawa","sequence":"additional","affiliation":[{"name":"Department of Agrobioscience Graduate School of Agricultural Science Kobe University  1\u20101 Rokkodai, Nada Kobe Hyogo 657\u20108501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,14]]},"reference":[{"key":"e_1_2_9_1_1","unstructured":"World Health Organization. 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