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Compare to the wild\u2010type (WT) strain, the \u0394aldB strain shows a smaller body size, grows more slowly, and contains more acid in the cell cytoplasm. The survival rate of the \u0394aldB strain is much lower in low pH and simulated gastric fluid (SGF) than that of the WT strain, but higher in simulated intestinal fluid (SIF). The antagonism test demonstrates the \u0394aldB strain can inhibit <jats:italic>Listeria monocytogenes<\/jats:italic> (<jats:italic>L. monocytogenes<\/jats:italic>) and <jats:italic>Salmonella<\/jats:italic> more effectively than the WT strain. Additionally, there is a dramatic decrease in the adhesion rate of <jats:italic>Salmonella<\/jats:italic> to Caco\u20102 and HT\u201029 cells in the presence of the \u0394aldB strain compared to the WT strain. Simultaneously analyze, the auto\u2010aggregation, co\u2010aggregation, cell surface hydrophobicity (CSH), hemolytic, temperature, NaCl, oxidative stress, and antibiotic susceptibility of the \u0394aldB strain are consistent with the features of probiotics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study highlights that the <jats:italic>aldB<\/jats:italic> gene plays a significant role in the growth and antibacterial properties of <jats:italic>L. reuteri<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202300337","type":"journal-article","created":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T21:25:05Z","timestamp":1701725105000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Acetolactate Decarboxylase as an Important Regulator of Intracellular Acidification, Morphological Features, and Antagonism Properties in the Probiotic <i>Lactobacillus reuteri<\/i>"],"prefix":"10.1002","volume":"68","author":[{"given":"Xueqing","family":"Wang","sequence":"first","affiliation":[{"name":"College of Chemistry &amp; 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