{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T19:51:35Z","timestamp":1789156295182,"version":"build-2803163510"},"reference-count":65,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T00:00:00Z","timestamp":1703721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p><jats:italic>Torreya grandis<\/jats:italic> kernel has traditionally been used to remove intestinal parasites and increases intestinal motility. However, the effect of <jats:italic>Torreya grandis<\/jats:italic> kernel oil (TKO) on constipation has not yet been investigated. Therefore, mouse model is used to investigate the effect of TKO on slow transit constipation (STC) and its possible mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>The effects of TKO on intestinal motility of STC mice are evaluated by fecal weight, fecal water content, colon length, defecation test, and intestinal propulsion test. The mechanism of TKO alleviating STC is explored by detecting biochemical analysis, histological analysis, western blot, qRT\u2010PCR, immunohistochemistry, and gut microbiota analysis. The results reveal that TKO effectively promotes defecation and intestinal motility, increases the level of endothelin\u20101, and restores the histopathological morphology of the colon under LOP pretreatment. The expression levels of occludin, claudin\u20101, and zonula occludens\u20101 (ZO\u20101) mRNA and protein are up\u2010regulated in mice receiving TKO treatment. The colonic 5\u2010hydroxytryptamine 3R\/4R (5\u2010HT3R\/5\u2010HT4R) expressions are also increased by TKO supplementation. Additionally, TKO rescues LOP\u2010caused disorders of the gut microbiota.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Consumption of TKO is beneficial to STC recovery, and it can alleviate LOP\u2010induced STC by up\u2010regulating the colonic expressions of Occludin\/Claudin\u20101\/ZO\u20101 and 5\u2010HT3R\/5\u2010HT4R.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202300615","type":"journal-article","created":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T10:15:11Z","timestamp":1703758511000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["<i>Torreya grandis<\/i> Kernel Oil Alleviates Loperamide\u2010Induced Slow Transit Constipation via Up\u2010Regulating the Colonic Expressions of Occludin\/Claudin\u20101\/ZO\u20101 and 5\u2010HT3R\/5\u2010HT4R in BALB\/c Mice"],"prefix":"10.1002","volume":"68","author":[{"given":"Xuezhu","family":"Wang","sequence":"first","affiliation":[{"name":"School of Public Health Zhejiang Chinese Medical University  Hangzhou Zhejiang 310053 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Public Health Zhejiang Chinese Medical University  Hangzhou Zhejiang 310053 China"},{"name":"Academy of Chinese Medical Science Zhejiang Chinese Medical University  Hangzhou Zhejiang 310053 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoli","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Public Health Zhejiang Chinese Medical University  Hangzhou Zhejiang 310053 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lalai","family":"Zikela","sequence":"additional","affiliation":[{"name":"School of Public Health Zhejiang Chinese Medical University  Hangzhou Zhejiang 310053 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaomei","family":"Li","sequence":"additional","affiliation":[{"name":"School of Public Health Zhejiang Chinese Medical University  Hangzhou Zhejiang 310053 China"},{"name":"Academy of Chinese Medical Science Zhejiang Chinese Medical University  Hangzhou Zhejiang 310053 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songtao","family":"Li","sequence":"additional","affiliation":[{"name":"School of Public Health Zhejiang Chinese Medical University  Hangzhou Zhejiang 310053 China"},{"name":"Academy of Chinese Medical Science Zhejiang Chinese Medical University  Hangzhou Zhejiang 310053 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1445-4682","authenticated-orcid":false,"given":"Qiang","family":"Han","sequence":"additional","affiliation":[{"name":"School of Public Health Zhejiang Chinese Medical University  Hangzhou Zhejiang 310053 China"},{"name":"Academy of Chinese Medical Science Zhejiang Chinese Medical University  Hangzhou Zhejiang 310053 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,28]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15586"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3949\/ccjm.84a.15141"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2019.01.031"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","unstructured":"J. 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