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However, the effect of allicin on glucose homeostasis in the state of insulin resistance remains to be elucidated. This study investigates the effect of allicin on glucose metabolism using different muscle fibers in a chicken model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Day\u2010old male Arbor Acres broilers are randomly divided into three groups and fed a basal diet supplemented with 0, 150, or 300\u00a0mg\u00a0kg<jats:sup>\u22121<\/jats:sup> allicin for 42 days. Results show that allicin improves the zootechnical performance of broilers at the finishing stage. The glucose loading test (2\u00a0g\u00a0kg<jats:sup>\u22121<\/jats:sup> body mass) indicates the regulatory role of allicin on glucose homeostasis. In vitro results demonstrate allicin increases glutathione (GSH) level and the expression of cystathionine \u03b3 lyase (CSE), leading to endogenous hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) production in <jats:italic>M. pectoralis major<\/jats:italic> (PM) muscle\u2010derived myotubes. Allicin stimulates adenosine monophosphate\u2010activated protein kinase (AMPK) S\u2010sulfhydration and AMPK phosphorylation to promote glucose uptake, which is suppressed in the presence of <jats:sc>d<\/jats:sc>,<jats:sc>l<\/jats:sc>\u2010propargylglycine (PAG, a CSE inhibitor).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrates that allicin induces AMPK S\u2010sulfhydration and AMPK phosphorylation to promote glucose uptake via the CSE\/H<jats:sub>2<\/jats:sub>S system in a muscle fiber\u2010dependent manner.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202300622","type":"journal-article","created":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T09:20:20Z","timestamp":1707556820000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Allicin Promotes Glucose Uptake by Activating AMPK through CSE\/H<sub>2<\/sub>S\u2010Induced S\u2010Sulfhydration in a Muscle\u2010Fiber Dependent Way in Broiler Chickens"],"prefix":"10.1002","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0944-3644","authenticated-orcid":false,"given":"Kelin","family":"Li","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province) Ministry of Agriculture and Rural Affairs Shandong Agricultural University Tai'an 271000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria A.","family":"Uyanga","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province) Ministry of Agriculture and Rural Affairs Shandong Agricultural University Tai'an 271000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province) Ministry of Agriculture and Rural Affairs Shandong Agricultural University Tai'an 271000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongchao","family":"Jiao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province) Ministry of Agriculture and Rural Affairs Shandong Agricultural University Tai'an 271000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingpeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province) Ministry of Agriculture and Rural Affairs Shandong Agricultural University Tai'an 271000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunlei","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Chemistry and Material Science Shandong Agricultural University Tai'an 271000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences Shandong Agricultural University Tai'an 271000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province) Ministry of Agriculture and Rural Affairs Shandong Agricultural University Tai'an 271000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,10]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11020295"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26061505"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101113"},{"key":"e_1_2_9_4_1","author":"Cheng B.","year":"2021","journal-title":"Evid. 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