{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T14:36:25Z","timestamp":1791815785686,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T00:00:00Z","timestamp":1712793600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007613","name":"Ankara Universitesi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007613","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Garlic is rich in bioactive compounds that are effective against colon cancer cells. This study tests the antioxidant and antiproliferative effects of cold\u2010extracted white and black garlic extracts. Black garlic extracted in water (SSU) exhibits the highest antioxidant activity, phenolic content, and flavonoid content, while black garlic extracted in ethanol (SET) shows the lowest values. Caspase\u20103 activity is notably higher in the white garlic extracted in methanol (BME), white garlic extracted in methanol combines with 5\u2010FU, black garlic extracted in ethanol (SET), black garlic extracted in ethanol combines with 5\u2010fluorouracil (5\u2010FU), and 5\u2010FU treatments compare to the control group (<jats:italic>p<\/jats:italic> &gt; 0.05). BME+5\u2010FU displays the highest caspase\u20108 activity (<jats:italic>p<\/jats:italic> &lt; 0.05). A decrease in NF\u2010\u03baB levels is observed in the SET+5\u2010FU group (<jats:italic>p<\/jats:italic>&gt;0.05), while COX\u20102 activities decrease in the BME, SET+5\u2010FU, SET, and 5\u2010FU groups (<jats:italic>p<\/jats:italic>&gt;0.05). Wound healing increases in the BME, BME+5\u2010FU, SET+5\u2010FU, and 5\u2010FU groups (<jats:italic>p<\/jats:italic> &lt; 0.05). In conclusion, aqueous black garlic extract may exhibit pro\u2010oxidant activity despite its high antioxidant capacity. It is worth noting that exposure to heat\u2010treated food and increased sugar content may lead to heightened inflammation and adverse health effects. This study is the first to combine garlic with chemo\u2010preventive drugs like 5\u2010FU in Caco\u20102 cells.<\/jats:p>","DOI":"10.1002\/mnfr.202300820","type":"journal-article","created":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T07:45:25Z","timestamp":1712821525000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Investigation of Antiproliferative Effects of Combinations of White and Black Garlic Extracts with 5\u2010Fluorouracil (5\u2010FU) on Caco\u20102 Colorectal Adenocarcinoma Cells"],"prefix":"10.1002","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0268-9812","authenticated-orcid":false,"given":"Derya","family":"Ozalp Unal","sequence":"first","affiliation":[{"name":"Republic of Turkey Ministry of Agriculture and Forestry Field Crops Central Research Institute  Ankara 06170 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tevhide","family":"Sel","sequence":"additional","affiliation":[{"name":"The Faculty of Veterinary Department of Biochemistry Ankara University  Ankara 06110 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-021-02099-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2014.0064"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2005.12.005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3923\/pjbs.2010.340.343"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2008.26142"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-9-135"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.4162\/nrp.2014.8.2.132"},{"key":"e_1_2_9_9_1","first-page":"725","volume":"61","author":"Shirin H.","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2017.01.001"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(01)00084-7"},{"key":"e_1_2_9_12_1","first-page":"8","volume-title":"Garlic and nutraceutical applications, Nutraceuticals","author":"Hismiogullar\u0131 S. E.","year":"2021"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038242"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5b01779"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5FO00120J"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.7.2171"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-008-9706-8"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.70434"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2012.04.009"},{"key":"e_1_2_9_20_1","first-page":"47","volume":"3","author":"Zhang D.","year":"2004","journal-title":"Chem"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.2217\/fon.15.235"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf9018382"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cogsc.2017.03.009"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2014.05.015"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2003.11.024"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-3501"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf500803c"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511005307"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11121828"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1128(199601)16:1<111::AID-MED4>3.0.CO;2-5"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1993.82"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1996.v110.pm8536847"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/01480540902862236"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.4081\/dr.2011.e4"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/DSS.0000000000001382"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.13220"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2014.10.005"},{"key":"e_1_2_9_38_1","first-page":"531","volume":"16","author":"Zainol M. K.","year":"2009","journal-title":"Int. Food Res. J."},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/jtxs.12354"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2021.105837"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2014.11.014"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-511X-13-195"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.06.011"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21040430"},{"key":"e_1_2_9_45_1","author":"Elango E.","year":"2004","journal-title":"Citeseer"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.202300820","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T15:56:09Z","timestamp":1718034969000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.202300820"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":44,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/mnfr.202300820"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.202300820","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"2023-11-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300820"}}