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In this regard, biofortification with microorganisms like <jats:italic>Bifidobacterium<\/jats:italic> and <jats:italic>Streptococcus<\/jats:italic> offers a compelling approach for enhancing food with natural folates. A randomized, nonblinded, and monocentric human pilot study is conducted to assess the bioavailability of a folate\u2010biofortified fermented whey beverage, comprising 3 intervention days and a controlled replenishment phase before and during the assay. Folate plasma concentration (5\u2010CH<jats:sub>3<\/jats:sub>\u2010H<jats:sub>4<\/jats:sub>folate) is determined using a stable isotope dilution assay and LC\u2010MS\/MS detection. Biokinetic parameters (<jats:italic>c<\/jats:italic><jats:sub>max<\/jats:sub> and <jats:italic>t<\/jats:italic><jats:sub>max<\/jats:sub>) are determined, and areas under the curve (AUC) normalized to the basal folate plasma concentration are calculated. An average bioavailability of 17.1% in relation to the 5\u2010CH<jats:sub>3<\/jats:sub>\u2010H<jats:sub>4<\/jats:sub>folate supplement, ranging from 0% to 39.8%, is obtained. These results reiterate the significance of additional research into folate bioavailability in general and dairy products. Further investigations are warranted into folate\u2010binding proteins (FBP) and other potential limiting factors within the food and individual factors. In summary, biofortification via fermentation emerges as a promising avenue for enhancing the natural folate content in dairy and other food products.<\/jats:p>","DOI":"10.1002\/mnfr.202300888","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T21:19:29Z","timestamp":1722633569000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Assessing a Fermented Whey Beverage Biofortified with Folate as a Potential Folate Source for Humans"],"prefix":"10.1002","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6289-9184","authenticated-orcid":false,"given":"Lisa","family":"Obermaier","sequence":"first","affiliation":[{"name":"Chair of Analytical Food Chemistry Technical University of Munich  85354 Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Clara","family":"Candelaria Cucick","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences University of Sao Paulo (USP)  S\u00e3o Paulo Brazil"},{"name":"Food Research Center University of S\u00e3o Paulo (USP)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernadette Dora","family":"Gombossy de Melo Franco","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences University of Sao Paulo (USP)  S\u00e3o Paulo Brazil"},{"name":"Food Research Center University of S\u00e3o Paulo (USP)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana Marta","family":"Isay Saad","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences University of Sao Paulo (USP)  S\u00e3o Paulo Brazil"},{"name":"Food Research Center University of S\u00e3o Paulo (USP)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6875-1658","authenticated-orcid":false,"given":"Thomas","family":"Skurk","sequence":"additional","affiliation":[{"name":"ZIEL Institute for Food &amp; Health Core Facility Human Studies Technical University of Munich  85354 Freising Germany"},{"name":"Technical University of Munich Klinikum rechts der Isar  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Rychlik","sequence":"additional","affiliation":[{"name":"Chair of Analytical Food Chemistry Technical University of Munich  85354 Freising Germany"},{"name":"Centre for Nutrition and Food Sciences University of Queensland  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,2]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.114.206599"},{"key":"e_1_2_10_2_1","first-page":"85","volume":"33","author":"Pfeiffer C.","year":"1994","journal-title":"J. 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