{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T03:50:06Z","timestamp":1773460206425,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,5,19]],"date-time":"2024-05-19T00:00:00Z","timestamp":1716076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Omega\u20103 fatty acids (O3FAs) and resveratrol (RSV) are known to be beneficial for certain eye diseases, such as age\u2010related macular degeneration (AMD). Neovascular AMD is characterized by abnormal blood vessel formation due to the excessive synthesis of vascular endothelial growth factor (VEGF) by retinal pigment epithelium (RPE) cells. The study investigates whether a formulation based on eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and RSV is capable of counteracting VEGF\u2010A secretion, and elucidates the molecular mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>The study finds, using ELISA, that O3FAs\/RSV reduces VEGF\u2010A secretion in human RPE cells. This phenomenon is related to the increased interaction between VEGF\u2010receptor 2 (VEGF\u2010R2) and caveolin\u20101 (CAV\u20101), a protein of detergent\u2010resistant membranes (DRMs), as determined by co\u2010immunoprecipitation and proximity ligation assay. Using microscale thermophoresis, the study confirms that O3FAs\/RSV causes a high\u2010affinity interaction. Isolation and analysis of DRMs reveal that this interaction is concomitant with VEGF\u2010R2 relocalization in DRMs. The depletion of DRMs by a cholesterol\u2010chelating agent blocks the VEGF\u2010R2\/CAV\u20101 interaction and EPA\/DHA\/RSV\u2010mediated impairment of VEGF production.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This specific interaction can provide a new strategy for countering VEGF\u2010A production in human RPE cells and, consequently, reducing neovascularization in AMD. Further preclinical studies involving O3FAs and polyphenols are warranted.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202300893","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T02:48:57Z","timestamp":1716173337000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["VEGF\u2010R2\/CAV\u20101 Interaction Induced by Resveratrol\/Eicosapentaenoic Acid\/Docosahexaenoic Acid\u2010Enriched Formulation through Functional Detergent\u2010Resistant Membranes Is Associated with Decreased VEGF\u2010A Release in ARPE\u201019 Cells"],"prefix":"10.1002","volume":"68","author":[{"given":"Maude","family":"Perus","sequence":"first","affiliation":[{"name":"UFR des Sciences de Sant\u00e9 Universit\u00e9 de Bourgogne  Dijon 21000 France"},{"name":"INSERM Research Center U1231\u2014Cancer and Adaptive Immune Response Team Bioactive Molecules and Health Research Group  Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavie","family":"Courtaut","sequence":"additional","affiliation":[{"name":"UFR des Sciences de Sant\u00e9 Universit\u00e9 de Bourgogne  Dijon 21000 France"},{"name":"INSERM Research Center U1231\u2014Cancer and Adaptive Immune Response Team Bioactive Molecules and Health Research Group  Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Paul","family":"Pais de Barros","sequence":"additional","affiliation":[{"name":"UFR des Sciences de Sant\u00e9 Universit\u00e9 de Bourgogne  Dijon 21000 France"},{"name":"INSERM Research Center U1231\u2014Cancer and Adaptive Immune Response Team Bioactive Molecules and Health Research Group  Dijon 21000 France"},{"name":"INSERM UMS58 BioSanD \u2010 Diviomic Platform  Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginie","family":"Aires","sequence":"additional","affiliation":[{"name":"UFR des Sciences de Sant\u00e9 Universit\u00e9 de Bourgogne  Dijon 21000 France"},{"name":"INSERM Research Center U1231\u2014Cancer and Adaptive Immune Response Team Bioactive Molecules and Health Research Group  Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5549-1992","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Hermetet","sequence":"additional","affiliation":[{"name":"UFR des Sciences de Sant\u00e9 Universit\u00e9 de Bourgogne  Dijon 21000 France"},{"name":"INSERM Research Center U1231\u2014Cancer and Adaptive Immune Response Team Bioactive Molecules and Health Research Group  Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8911-8499","authenticated-orcid":false,"given":"Dominique","family":"Delmas","sequence":"additional","affiliation":[{"name":"UFR des Sciences de Sant\u00e9 Universit\u00e9 de Bourgogne  Dijon 21000 France"},{"name":"INSERM Research Center U1231\u2014Cancer and Adaptive Immune Response Team Bioactive Molecules and Health Research Group  Dijon 21000 France"},{"name":"INSERM UMS58 BioSanD \u2010 Diviomic Platform  Dijon 21000 France"},{"name":"Centre de Lutte Contre le Cancer Georges Fran\u00e7ois Leclerc  Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,19]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2021.153554"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200400098"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2019.111091"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10111677"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10111743"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22031295"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(13)70145-1"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/cge.12206"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03420-x"},{"key":"e_1_2_9_10_1","first-page":"5397","volume":"9","author":"Ba J.","year":"2015","journal-title":"Drug Des. 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