{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:17:07Z","timestamp":1791908227130,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T00:00:00Z","timestamp":1718064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100014472","name":"Scientific Research Foundation of Hunan Provincial Education Department","doi-asserted-by":"publisher","award":["22B0406"],"award-info":[{"award-number":["22B0406"]}],"id":[{"id":"10.13039\/100014472","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014472","name":"Scientific Research Foundation of Hunan Provincial Education Department","doi-asserted-by":"publisher","award":["20C1603"],"award-info":[{"award-number":["20C1603"]}],"id":[{"id":"10.13039\/100014472","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Polycystic ovary syndrome (PCOS) is closely related to non\u2010alcoholic fatty liver disease (NAFLD), and sex hormone\u2010binding globulin (SHBG) is a glycoprotein produced by the liver. Hepatic lipogenesis inhibits hepatic SHBG synthesis, which leads to hyperandrogenemia and ovarian dysfunction in PCOS. Therefore, this study aims to characterize the mechanism whereby liver lipogenesis inhibits SHBG synthesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>This study establishes a rat model of PCOS complicated by NAFLD using a high\u2010fat diet in combination with letrozole and performs transcriptomic analysis of the liver. Transcriptomic analysis of the liver shows that the expression of neurite growth inhibitor\u2010B receptor (NgBR), hepatocyte nuclear factor 4\u03b1 (HNF4\u03b1), and SHBG is low. Meantime, HepG2 cells are treated with palmitic acid (PA) to model NAFLD in vitro, which causes decreases in the expression of NgBR, HNF4\u03b1, and SHBG. However, the expression of HNF4\u03b1 and SHBG is restored by treatment with the AMP\u2010activated protein kinase (AMPK) agonist AICAR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>NgBR regulates the expression of HNF4\u03b1 by activating the AMPK signaling pathway, thereby affecting the synthesis of SHBG in the liver. Further mechanistic studies regarding the effect of liver fat on NGBR expression are warranted.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202300915","type":"journal-article","created":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T01:43:46Z","timestamp":1718156626000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Hyperglycemia Inhibits Hepatic SHBG Synthesis Through the NGBR\u2010AMPK\u2010HNF4 Pathway in Rats with Polycystic Ovary Syndrome Induced by Letrozole in Combination with a High\u2010Fat Diet"],"prefix":"10.1002","volume":"68","author":[{"given":"Rao","family":"Hu","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital, Gynecology &amp;Obstetrics and Reproductive Medical Center, Hengyang Medical School University of South China  Hengyang Hunan 421001 China"},{"name":"Institute of Applied Anatomy and Reproductive Medicine, Hengyang Medical College University of South China  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