{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T16:33:31Z","timestamp":1783787611459,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2024,10,19]],"date-time":"2024-10-19T00:00:00Z","timestamp":1729296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["INST 370\/1\u20101 FUGG"],"award-info":[{"award-number":["INST 370\/1\u20101 FUGG"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Excessive activation of the nucleotide\u2010binding oligomerization domain\u2010like receptor pyrin domain\u2010containing protein 3 (NLRP3) inflammasome contributes to chronic inflammation. Thus, targeting NLRP3 inflammasome activation by anthocyanins may prevent inflammatory diseases. Therefore, the present study determines the influence of a black carrot extract (BCE) with high amounts of acylated anthocyanins and their related phenolic acids on the NLRP3 inflammasome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>THP\u20101 monocytes are pretreated with a BCE, cyanidin\u20103\u2010glucoside (C3G), or hydroxycinnamic acids. NLRP3 inflammasome assembly is initiated by priming THP\u20101 monocytes with lipopolysaccharide and\/or activating the NLRP3 inflammasome with nigericin. Flow cytometry is used to assess apoptosis\u2010associated speck\u2010like protein containing a caspase recruitment domain (ASC) speck formation, as well as ASC and NLRP3 protein expression. Caspase\u20101 activity is measured using a bioluminescent assay, and cytokine concentrations are determined by enzyme\u2010linked immunosorbent assays (ELISA). C3G and phenolic acids diminish ASC and NLRP3 protein expression. In addition, C3G and phenolic acids attenuate ASC speck formation. Furthermore, the BCE and C3G decline caspase\u20101 activity. Consistently, IL\u20101\u03b2 and IL\u201018 secretion are reduced upon NLRP3 inflammasome activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The present study shows that a BCE with high amounts of acylated anthocyanins and their related phenolic acids diminish priming and activation of the NLRP3 inflammasome in THP\u20101 monocytes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202400356","type":"journal-article","created":{"date-parts":[[2024,10,19]],"date-time":"2024-10-19T09:15:07Z","timestamp":1729329307000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Acylated Anthocyanins From Black Carrots and Their Related Phenolic Acids Diminish Priming and Activation of the NLRP3 Inflammasome in THP\u20101 Monocytes"],"prefix":"10.1002","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4492-3774","authenticated-orcid":false,"given":"Inken","family":"Behrendt","sequence":"first","affiliation":[{"name":"Institute of Nutritional Science, Department of Nutritional Science Justus\u2010Liebig\u2010University Giessen  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Becker","sequence":"additional","affiliation":[{"name":"Chair of Analysis and Technology of Plant\u2010based Foods \u2013 Focus on Beverages Department of Beverage Research Geisenheim University  Geisenheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8269-4525","authenticated-orcid":false,"given":"Christof Bj\u00f6rn","family":"Steingass","sequence":"additional","affiliation":[{"name":"Chair of Analysis and Technology of Plant\u2010based Foods \u2013 Focus on Beverages Department of Beverage Research Geisenheim University  Geisenheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Schweiggert","sequence":"additional","affiliation":[{"name":"Chair of Analysis and Technology of Plant\u2010based Foods \u2013 Focus on Beverages Department of Beverage Research Geisenheim University  Geisenheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Michel","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology, Transfusion Medicine and Hemostaseology, Department of Medicine Justus\u2010Liebig\u2010University Giessen  Giessen Germany"},{"name":"Flow Cytometry Core Facility Department of Medicine Justus\u2010Liebig\u2010University Giessen  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvira","family":"Friedrich","sequence":"additional","affiliation":[{"name":"Institute of Nutritional Science, Department of Nutritional Science Justus\u2010Liebig\u2010University Giessen  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Grote","sequence":"additional","affiliation":[{"name":"Institute of Nutritional Science, Department of Nutritional Science Justus\u2010Liebig\u2010University Giessen  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"Martin","sequence":"additional","affiliation":[{"name":"Institute of Nutritional Science, Department of Nutritional Science Justus\u2010Liebig\u2010University Giessen  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna Pauline","family":"D\u00f6tzer","sequence":"additional","affiliation":[{"name":"Institute of Nutritional Science, Department of Nutritional Science Justus\u2010Liebig\u2010University Giessen  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Fasshauer","sequence":"additional","affiliation":[{"name":"Institute of Nutritional Science, Department of Nutritional Science Justus\u2010Liebig\u2010University Giessen  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Speckmann","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology, Transfusion Medicine and Hemostaseology, Department of Medicine Justus\u2010Liebig\u2010University Giessen  Giessen Germany"},{"name":"Flow Cytometry Core Facility Department of Medicine Justus\u2010Liebig\u2010University Giessen  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Kuntz","sequence":"additional","affiliation":[{"name":"Institute of Nutritional Science, Department of Nutritional Science Justus\u2010Liebig\u2010University Giessen  Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,19]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"crossref","unstructured":"D.Zheng T.Liwinski E.Elinav Cell Discov2020 6 36.","DOI":"10.1038\/s41421-020-0167-x"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14102103"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02538"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-016-2396-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124294"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-071816-064836"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12060852"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph16050638"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b06737"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf104724k"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541-4337.12836"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf060300l"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf020690l"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29030691"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf050570o"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes11080906"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c05879"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf802988s"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-005-0557-8"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.202000898"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1079\/NRR2005116"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2015.08.011"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo14040203"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2014.08.002"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-021-00500-4"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11130-013-0371-z"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.565924"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1401110"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2017.03.004"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0168610"},{"key":"e_1_2_9_31_1","first-page":"174","volume":"71","author":"Greevenbroek M. M. J.","year":"2013","journal-title":"Neth. J. Med."},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S141220"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.08.006"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2016.02.004"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-023-02743-8"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13273-021-00219-5"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2022.109204"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10112880"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10092327"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2020.105637"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22081315"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S277457"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2014.04.033"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00071"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2022.101383"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.03.130"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.202400356","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T12:12:11Z","timestamp":1732882331000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.202400356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,19]]},"references-count":46,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/mnfr.202400356"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.202400356","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,19]]},"assertion":[{"value":"2024-05-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2400356"}}