{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:54:48Z","timestamp":1791608088638,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2024,11,3]],"date-time":"2024-11-03T00:00:00Z","timestamp":1730592000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472955"],"award-info":[{"award-number":["32472955"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302795"],"award-info":[{"award-number":["32302795"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003352","name":"Beijing University of Agriculture","doi-asserted-by":"publisher","award":["BUA\u2010HHXD2022008"],"award-info":[{"award-number":["BUA\u2010HHXD2022008"]}],"id":[{"id":"10.13039\/501100003352","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Aging in males can lead to declines in testosterone production, essential for maintaining male reproductive health.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>To investigate the effects of dietary supplementation with alpha\u2010linolenic acid (ALA) on testosterone production in aged breeder roosters and understand the underlying molecular mechanisms involved. An in vivo model is established to investigate the effects of dietary ALA supplementation on testosterone production in aged breeder roosters, and the Leydig cell culture is used to identify the potential molecular mechanism. Dietary supplementation with ALA increases in plasma testosterone. Congruently, ALA supplementation enhances the expression of testosterone biosynthesis\u2010related enzymes. ALA supplementation exerts anti\u2010apoptotic effects in testicular mitochondria, as evidenced by a lower expression of pro\u2010apoptotic factors and a higher expression of the anti\u2010apoptotic factor B\u2010cell lymphoma 2 (Bcl\u20102). Moreover, In Leydig cells, ALA supplementation promotes mitochondrial biogenesis genes. The proposed mechanism is that ALA activates the sirtuin1 (SIRT1)\u00a0pathway and is supported by higher SIRT1 transcript and protein in Leydig cells. Furthermore, blocking SIRT1 with siRNA reverses ALA's effects on testosterone biosynthesis and mitochondrial function\u2010related genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings indicate that dietary supplementation with ALA can improve testosterone production in aged breeder roosters, possibly by modulation of mitochondrial function via activating the SIRT1 pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202400522","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T06:17:43Z","timestamp":1730701063000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Alpha\u2010Linolenic Acid Supplementation Improves Testosterone Production in an Aged Breeder Rooster Model: Role of Mitochondrial Modulation and SIRT1 Activation"],"prefix":"10.1002","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6603-6685","authenticated-orcid":false,"given":"Cheng","family":"Long","sequence":"first","affiliation":[{"name":"Animal Science and Technology College Beijing University of Agriculture  Beijing 102206 China"},{"name":"Department of Agricultural, Food and Nutritional Science University of Alberta  Edmonton AB T6G 2P5 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010xian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Animal Science and Technology College Beijing University of Agriculture  Beijing 102206 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin P.","family":"Willing","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science University of Alberta  Edmonton AB T6G 2P5 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi\u2010Hui","family":"Sheng","sequence":"additional","affiliation":[{"name":"Animal Science and Technology College Beijing University of Agriculture  Beijing 102206 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang\u2010Guo","family":"Wang","sequence":"additional","affiliation":[{"name":"Animal Science and Technology College Beijing University of Agriculture  Beijing 102206 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long\u2010Fei","family":"Xiao","sequence":"additional","affiliation":[{"name":"Animal Science and Technology College Beijing University of Agriculture  Beijing 102206 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Long","family":"Qi","sequence":"additional","affiliation":[{"name":"Animal Science and Technology College Beijing University of Agriculture  Beijing 102206 China"},{"name":"Key Laboratory of Agricultural Product Processing and Quality Control (Co\u2010construction by Ministry and Province) Ministry of Agriculture and Rural Affairs  Beijing 102206 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,3]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.03.016"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.843800"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2003-0030"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2014.03.016"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2023.06.030"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1009176107"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.B1700148"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/md19040182"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1427.006"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.026"},{"key":"e_1_2_10_11_1","volume-title":"Reproductive Biology and Phylogeny of Birds: Phylogeny, Morphology, Hormones, Fertilization (Volume 6A of Series)","author":"Bock W. J.","year":"2007"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2952"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11091684"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.42387"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0226769"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8080928"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.78654"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-020-03312-8"},{"key":"e_1_2_10_19_1","first-page":"102","volume":"41","author":"Li W.","year":"2019","journal-title":"J. Jilin Agric. Univ."},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.14348\/molcells.2016.2318"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2019.135077"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19113447"},{"key":"e_1_2_10_23_1","first-page":"195","volume":"17","author":"Ye G.","year":"2019","journal-title":"Oncol. Lett."},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2017.1295182"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.1859"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/15376516.2017.1354413"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deaa153"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.01225"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7290"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrestox.8b00201"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-1599-3_6"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11573"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-019-0158-9"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24173084"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioab150"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.163667"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.911714"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.B1500158"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2023.06.030"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2020.213419"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.202400522","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T12:12:47Z","timestamp":1732882367000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.202400522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":40,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/mnfr.202400522"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.202400522","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"2024-07-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2400522"}}