{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:51:25Z","timestamp":1788886285029,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T00:00:00Z","timestamp":1730160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1600805"],"award-info":[{"award-number":["2022YFD1600805"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Scope<\/jats:title><jats:p>Acute alcoholic liver injury (AALI), a global health concern, is exacerbated by excessive episodic drinking. L\u2010theanine (LTA), a compound found in tea leaves, mitigates the AALI\u2010induced liver oxidative stress and inflammation. However, its relationship with alcohol metabolism and its liver\u2010protective mechanism remains unexplored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>This study investigates the protective mechanisms of LTA against AALI in mice. The results demonstrate that LTA mitigates liver tissue damage and reduces the serum levels of aspartate aminotransferase and alanine aminotransferase, and liver levels of triglycerides, malondialdehyde, reactive oxygen species (ROS), tumor necrosis factor\u2010\u03b1, interleukin\u20106, and interleukin\u20101\u03b2. However, LTA enhances the activity of ethanol\u2010metabolizing enzymes and decreases ethanol and acetaldehyde serum levels. Mechanistically, LTA accelerates alcohol metabolism by upregulating the hepatic expression of ADH6, ALDH1B1, ALDH2, CAT, and ACSS1 mRNA and protein in AALI mice, LTA downregulates the expression of CYP2E1 mRNA and protein and promoting antioxidative activities thus reducing the accumulation of ROS. This attenuated inflammation by inhibiting the phosphorylation of nuclear factor\u2010kappa B inhibitor alpha (I\u03baB\u03b1) and downregulating the hepatic expression of NF\u2010\u03baB p65, TNF\u2010\u03b1, IL\u20101\u03b2, IL\u20106 mRNA, and protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>LTA is a beneficial dietary supplement that protects against AALI by modulating alcohol metabolism and the TNF\u2010\u03b1\/NF\u2010\u03baB pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.202400766","type":"journal-article","created":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T08:26:59Z","timestamp":1730190419000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Protective Effect and Mechanism of <scp>L<\/scp>\u2010Theanine on Acute Alcoholic Liver Injury in Mice"],"prefix":"10.1002","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0124-5677","authenticated-orcid":false,"given":"Meng\u2010Yuan","family":"Liu","sequence":"first","affiliation":[{"name":"Key Lab of Tea Science of Ministry of Education Hunan Agricultural University  Changsha Hunan 410128 China"},{"name":"National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients Hunan Agricultural University  Changsha Hunan 410128 China"},{"name":"Sino\u2010Kenya Joint Laboratory of Tea Science Hunan Agricultural University  Changsha Hunan 410128 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai\u2010Hang","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Lab of Tea Science of Ministry of Education Hunan Agricultural University  Changsha Hunan 410128 China"},{"name":"National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients Hunan Agricultural University  Changsha Hunan 410128 China"},{"name":"Sino\u2010Kenya Joint Laboratory of Tea Science Hunan Agricultural University  Changsha Hunan 410128 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Lab of Tea Science of Ministry of Education Hunan Agricultural University  Changsha Hunan 410128 China"},{"name":"National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients Hunan Agricultural University  Changsha Hunan 410128 China"},{"name":"Sino\u2010Kenya Joint Laboratory of Tea Science Hunan Agricultural University  Changsha Hunan 410128 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6258-4414","authenticated-orcid":false,"given":"Wen\u2010Jun","family":"Xiao","sequence":"additional","affiliation":[{"name":"Key Lab of Tea Science of Ministry of Education Hunan Agricultural University  Changsha Hunan 410128 China"},{"name":"National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients Hunan Agricultural University  Changsha Hunan 410128 China"},{"name":"Sino\u2010Kenya Joint Laboratory of Tea Science Hunan Agricultural University  Changsha Hunan 410128 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,29]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-023-02383-8"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00041.2014"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23258"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000447820"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389200217666160125113806"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4161\/oxim.3.3.12288"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.11.010"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.848918"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.110.034678"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.580167"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23020774"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22158221"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01111.x"},{"key":"e_1_2_9_14_1","first-page":"2070","volume":"278","author":"Fan C.","year":"2003","journal-title":"J. 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