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Considering SMCSO is found at a higher abundance compared to the glucosinolates, there are limited reports on its effect on health, with the majority of the evidence on the beneficial effects on glucose metabolism in rodent models. In the current study, we investigated the metabolic effects of SMCSO and its metabolite, S\u2010methyl methanethiosulfonate (MMTSO), on prostate cancer metabolism. DU145 prostate cancer cells were cultured in 5.5\u00a0mM (basal), 10\u00a0mM (intermediate) and 25\u00a0mM (high) glucose concentrations in the presence of SMCSO or MMTSO (100\u00a0\u00b5M). Using Seahorse technology, MMTSO but not SMCSO reduced mitochondrial metabolism, mitochondrial ATP, and the percentage of oxidative phosphorylation and increased the fatty acid dependency of DU145 cells. Transcriptomic and metabolomic analyses observed cellular and energy metabolism pathways and immune response changes.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>These data show that MMTSO alters several features of energy metabolism in DU145 prostate cancer cells, shifting them towards a non\u2010cancerous phenotype. These data are consistent with the notion that MMTSO may contribute to the beneficial effects of a broccoli\u2010rich diet and metabolic effects of prostate cancer.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.70008","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T06:05:57Z","timestamp":1741759557000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["S\u2010Methyl Methanethiosulfonate, the Main Human Metabolite of S\u2010Methyl\u2010L\u2010Cysteine Sulfoxide, Alters Energy Metabolism in Prostate Cancer Cells"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1460-3679","authenticated-orcid":false,"given":"Gemma","family":"Beasy","sequence":"first","affiliation":[{"name":"Quadram Institute Bioscience  Norwich 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