{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T06:02:49Z","timestamp":1763445769197,"version":"3.41.2"},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T00:00:00Z","timestamp":1742688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Abdominal aortic aneurysm (AAA) is a local enlargement of the abdominal aorta with the risk of its fatal rupture. With lack of established medical treatments reducing the AAA growth, the development of treatment strategies for AAA is warranted. In this study, we examined whether aged garlic extract (AGE), which reportedly has vasoprotective effects, attenuates AAA formation in an animal model. AAAs were induced via CaCl<jats:sub>2<\/jats:sub> application to the abdominal aortas of C57BL\/6J mice. The intake of an AGE\u2010containing diet attenuated the aortic dilatation after AAA induction. Histological analysis showed that the degradation of elastic fibers in AAAs was mild in mice fed AGE compared to that in control mice. In addition, the expression of matrix metalloproteinase 9 (MMP9), a proteolytic enzyme degrading aortic tissue, was decreased in AAAs from mice fed with AGE. In cultured macrophages, AGE significantly inhibited tumor necrosis factor (TNF)\u2010\u03b1\u2010induced <jats:italic>Mmp9<\/jats:italic> mRNA expression. Furthermore, AGE attenuated TNF\u2010\u03b1\u2010induced extracellular signal\u2010regulated kinase (ERK) phosphorylation and <jats:italic>c\u2010fos<\/jats:italic> mRNA expression, suggesting the suppressive effect of AGE on ERK\u2010c\u2010Fos\u2010MMP9 axis induced by TNF\u2010\u03b1. The present findings indicate that AGE diminishes AAA formation by mitigating tissue destruction, highlighting the potential cardiovascular benefits of AGE intake.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.70028","type":"journal-article","created":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T08:36:22Z","timestamp":1742805382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Aged Garlic Extract Attenuates CaCl<sub>2<\/sub>\u2010Induced Abdominal Aortic Aneurysm Formation by Inhibiting Matrix Metalloproteinase 9 Expression in Macrophages"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3845-828X","authenticated-orcid":false,"given":"Kohei","family":"Karasaki","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Physiology and Medicine, Graduate School of Biomedical and Health Sciences Hiroshima University  Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari","family":"Ishida","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Physiology and Medicine, Graduate School of Biomedical and Health Sciences Hiroshima University  Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Kokubo","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Physiology and Medicine, Graduate School of Biomedical and Health Sciences Hiroshima University  Hiroshima Japan"},{"name":"Department of Physical Therapy Tohto University  Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiemi","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Physiology and Medicine, Graduate School of Biomedical and Health Sciences Hiroshima University  Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, Graduate School of Biomedical and Health Sciences Hiroshima University  Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masao","family":"Yoshizumi","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Physiology and Medicine, Graduate School of Biomedical and Health Sciences Hiroshima University  Hiroshima Japan"},{"name":"Faculty of Nursing Yasuda Women's University  Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcad.2021.03.009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehad386"},{"key":"e_1_2_9_4_1","first-page":"1574","article-title":"Chemistry of Aged Garlic: Diversity of Constituents in Aged Garlic Extract and Their Production Mechanisms via the Combination of Chemical and Enzymatic Reactions","volume":"19","author":"Kodera Y.","year":"2020","journal-title":"Experimental and Therapeutic Medicine"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/136.3.769S"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-01866-3"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/1358863X211021170"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073877"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2013.176"},{"key":"e_1_2_9_10_1","first-page":"1490","article-title":"Effects of Aged Garlic Extract on Arterial Elasticity in a Placebo\u2010Controlled Clinical Trial Using EndoPAT\u2122 Technology","volume":"19","author":"Gruenwald J.","year":"2020","journal-title":"Experimental and Therapeutic Medicine"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147088"},{"key":"e_1_2_9_12_1","unstructured":"United States Pharmacopeial Convention United States Pharmacopeia 38 Garlic Fluid Extract USP 28\u2010NF 33(2015) 6052\u20136055."},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2023.1248561"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20948"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2308"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2006.11.020"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803164"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.116.308542"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.15581"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25809"},{"key":"e_1_2_9_21_1","first-page":"F123","article-title":"Dual Regulation of IL\u20101 Beta\u2010Mediated Matrix Metalloproteinase\u20109 Expression in Mesangial Cells by NF\u2010kappa B and AP\u20101","volume":"270","author":"Yokoo T.","year":"1996","journal-title":"American Journal of Physiology"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(97)90026-3"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2012.09.010"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.12945\/j.aorta.2013.13-052"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2013.03.009"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/10409230290771546"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2015.01.016"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19763-0"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvab179"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12872-021-02193-1"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-44523-6"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215334"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0171860"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.10.155"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2011.0099"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0284713"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00029.2013"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13402-020-00549-x"},{"key":"e_1_2_9_39_1","first-page":"581","article-title":"Macrophages in Rheumatoid Arthritis","volume":"22","author":"Maruotti N.","year":"2007","journal-title":"Histology and Histopathology"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10255"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.12.6244"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamcollsurg.2012.06.414"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-018-1851-z"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C500353200"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.70028","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T02:11:39Z","timestamp":1749435099000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.70028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,23]]},"references-count":43,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/mnfr.70028"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.70028","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"type":"print","value":"1613-4125"},{"type":"electronic","value":"1613-4133"}],"subject":[],"published":{"date-parts":[[2025,3,23]]},"assertion":[{"value":"2024-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70028"}}