{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:17:16Z","timestamp":1788481036230,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2022QH177"],"award-info":[{"award-number":["ZR2022QH177"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Epithelial barrier dysfunction, critically involved in intestinal ischemia\/reperfusion (I\/R) injury, is significantly regulated by Nrf2\u2010mediated oxidative stress. Apigenin, a flavonoid commonly found in fruits and vegetables with diverse biological properties, has an unclear impact on intestinal I\/R injury. We hypothesize that apigenin improves intestinal barrier dysfunction by activating Nrf2 signaling. Thirty rats were randomly divided into five groups to establish an I\/R model using superior mesenteric artery occlusion. Hypoxia and re\u2010oxygenation (H\/R) model was developed utilizing Caco\u20102 and IEC\u20106 cells, which were exposed to hypoxic conditions followed by re\u2010oxygenation. Apigenin protected against intestinal mucosal damage by suppressing inflammatory cytokines release (TNF\u2010\u03b1, IL\u20101\u03b2, IL\u20106, MPO,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.01), ameliorating oxidative stress (MDA, SOD, GSH, GSH\u2010Px,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.01), and improving barrier dysfunction (DAO and TEER,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.01) both in vivo and in vitro, without causing significant changes in the corresponding normal controls (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&gt;\u00a00.05). Apigenin up\u2010regulated the protein expression of Nrf2, HO\u20101, and tight junction (TJ) proteins (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.01). Furthermore, the knockdown of Nrf2 significantly abrogated apigenin\u2010enhanced the TJ expression. Apigenin pretreatment alleviates intestinal I\/R\u2010induced barrier damage through Nrf2 activation and TJ upregulation, offering new strategies for preventing or treating I\/R\u2010associated intestinal diseases.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70043","type":"journal-article","created":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T11:35:43Z","timestamp":1743334543000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Apigenin Alleviates Intestinal Ischemia\/Reperfusion Injury via Upregulating Nrf2\u2010Mediated Tight Junction Integrity"],"prefix":"10.1002","volume":"69","author":[{"given":"Bin","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Physiology Zhejiang Chinese Medical University  Hangzhou Zhejiang China"},{"name":"Key Laboratory of Biomedical Engineering &amp; Technology Qilu Medical University  Zibo Shandong China"},{"name":"Department of Pathology The First Affiliated Hospital Zhejiang University School of Medicine  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomedical Engineering &amp; Technology Qilu Medical University  Zibo Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Environmental Physiology Faculty of Medicine Shimane University  Shimane Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suoning","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gastric and Colorectal Surgery General Surgery Center The First Hospital of Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongjun","family":"Fan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomedical Engineering &amp; Technology Qilu Medical University  Zibo Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3538-3666","authenticated-orcid":false,"given":"Weiru","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Physiology Zhejiang Chinese Medical University  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111482"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(22)00241-2"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-023-00890-0"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu8080502"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2021.02.007"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00914"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2020.07.003"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202201540R"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eng.2023.11.016"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-024-01891-x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2023.176090"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3FO03669C"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2014.05.019"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-017-0798-6"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2021.112746"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15574"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27092901"},{"issue":"16","key":"e_1_2_9_19_1","first-page":"9139","article-title":"Apigenin Suppresses MED28\u2010Mediated Cell Growth in Human Liver Cancer Cells","volume":"72","author":"Chou J. 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