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This study investigates the intestinal microbiota characteristics in CC and FC patients, revealing microbial imbalances characterized by reduced beneficial taxa, such as <jats:italic>Acinetobacter, Blautia, Dorea formicigenerans, Eubacterium ramulus<\/jats:italic>, and <jats:italic>Halomonas<\/jats:italic>, alongside increased levels of <jats:italic>Alistipes, Holdemanella, Parabacteroides, Pseudomonas, Streptococcus<\/jats:italic>, and so on. These findings highlight dysbiosis as a critical factor in constipation pathogenesis and provide a foundation for the bottom\u2010up design of targeted probiotics and prebiotics. Potential therapeutic strategies include tailored probiotic formulations to replenish deficient taxa and the application of prebiotics to restore microbial balance. Additionally, the role of microbial metabolites, particularly short\u2010chain fatty acids, and the microbiota\u2013gut\u2013brain axis offers further insight into mechanisms underlying symptom modulation. 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