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Gut dysfunction and dysbiosis contribute to age\u2010related health decline. Grape seed\u2010derived procyanidins (GSPE) have shown efficacy in mitigating adaptive homeostasis decline in young animal models, but their impact on intestinal health and the gut microbiome in aged animals remains unexplored. Twenty\u2010one\u2010month\u2010old female rats were treated with 500\u00a0mg GSPE\/kg of body weight for 10 days. After 11 weeks, GSPE anti\u2010aging potential was evaluated by measuring plasma lipopolysaccharide, gut integrity gene expression, ex vivo gut barrier function, myeloperoxidase activity, and fecal microbiome composition. GSPE shifted the microbiota toward a younger profile, even restoring lost strains in aged rats. Despite the presence of metabolic aging markers, there was minimal deterioration in gut barrier function. Neither ex vivo permeability tests, transcriptional analysis of barrier function, nor gut histology showed significant impairment in gut. Only jejunal myeloperoxidase activity was increased in aged rats and reduced by GSPE. Intestinal barrier function showed mild deterioration in this model of aged rats. GSPE improved the aging process by modulating the gut microbiome, suggesting its potential as a microbiome\u2010targeted intervention for promoting healthy aging.<\/jats:p>","DOI":"10.1002\/mnfr.70150","type":"journal-article","created":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T02:50:33Z","timestamp":1750301433000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Grape Seed Proanthocyanidins: A Potential Microbiome\u2010Targeted Intervention for Healthy Aging in Rats"],"prefix":"10.1002","volume":"69","author":[{"given":"Marta","family":"Sierra\u2010Cruz","sequence":"first","affiliation":[{"name":"Universitat Rovira i Virgili, MoBioFood Research Group, Departament de Bioqu\u00edmica i Biotecnologia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adri\u00e0","family":"Vilalta","sequence":"additional","affiliation":[{"name":"Universitat Rovira i Virgili, MoBioFood Research Group, Departament 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