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Despite honey bees (<jats:italic>Apis mellifera<\/jats:italic>) being widely distributed worldwide, <jats:italic>Lepidotrigona<\/jats:italic> sp. is a particular genuine stingless bee that can be found only in the Southeast Asia region and Taiwan. Hence, the objectives of this study were firstly to identify <jats:italic>Lepidotrigona<\/jats:italic> propolis as an alternative medicine and investigate the anti\u2010inflammatory effects of on lipopolysaccharide (LPS) induced RAW 264.7 macrophage cells by the determination of cell viability, dendritic cell morphology, nitric oxide (NO) production, reactive oxygen species (ROS) detection, and inflammation\u2010related proteins analysis. In this study, the polyphenols and flavones in the propolis have been detected by UPLC\u2010ESI\u2010MS\/MS. Compared to the LPS group, this propolis type also showed decreased NO and ROS production and a change in cell morphology. Additionally, <jats:italic>Lepidotrigona<\/jats:italic> propolis can downregulate the expression of some inflammation\u2010related proteins, such as iNOS, TNF\u2010\u03b1, NF\u2010\u03baB, and IL\u20106. In summary, our study has unveiled the diverse therapeutic properties of <jats:italic>Lepidotrigona<\/jats:italic> propolis, particularly its potent anti\u2010inflammatory effects. This discovery opens up a promising avenue for developing a novel therapeutic alternative for human health, instilling hope for the future of medical science.<\/jats:p>","DOI":"10.1002\/mnfr.70163","type":"journal-article","created":{"date-parts":[[2025,7,13]],"date-time":"2025-07-13T11:58:49Z","timestamp":1752407929000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Functional Ability of Stingless Bees (<i>Lepidotrigona<\/i>) Propolis on Inflammation\u2010Related Disorders"],"prefix":"10.1002","volume":"69","author":[{"given":"Supawan","family":"Sunthorn","sequence":"first","affiliation":[{"name":"Department of Food Science College of Agriculture National Pingtung University of Science and Technology Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Chao","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science and Environmental Biology Kaohsiung Medical University Kaohsiung City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Hui","family":"Chiu","sequence":"additional","affiliation":[{"name":"Research Center for Food and Cosmetic Safety College of Human Ecology Chang Gung University of Science and Technology Taoyuan Taiwan"},{"name":"Department of Nephrology Chang Gung Memorial Hospital, Linkou Branch Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2555-6198","authenticated-orcid":false,"given":"Yung\u2010Lin","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Food Science College of Agriculture National Pingtung University of Science and Technology Pingtung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c04120"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3897\/zookeys.1172.104944"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0308-8146(03)00216-4"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00412"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1590\/1809-4392201501045"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2019.108756"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/127672"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4103\/1947-2714.100998"},{"key":"e_1_2_8_10_1","article-title":"Flow Cytometric Measurement of ROS Production in Macrophages in Response to Fc\u03b3R Cross\u2010Linking","volume":"145","author":"Shehat M. 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